中国医科大学学报2024，Vol.53Issue(3)：235-239,5.DOI:10.12007/j.issn.0258-4646.2024.03.008
褪黑素对人腹膜间皮细胞上皮-间质转化的作用及机制
Effect and mechanism of melatonin on the epithelial-mesenchymal transition of human peritoneal mesenchymal cells
摘要
Abstract
Objective To investigate the effect and mechanism of melatonin on the epithelial-mesenchymal transition(EMT)of human peritoneal mesothelial cells.Methods A human peritoneal mesoepithelial cell line(HMrSV5)was cultured.Decorin(DCN)overex-pression and knockout plasmids were constructed.The cells were divided into the normal,TGF-β1,TGF-β1+melatonin,TGF-β1+mela-tonin+siDCN,TGF-β1+melatonin+siNC,and TGF-β1+DCN groups.The cell proliferation and survival rates were determined using Cell Counting Kit-8(CCK-8).The protein and mRNA expression levels of DCN,TGF-β1,Smad2,and E-cadherin were detected by Western blotting and real-time polymerase chain reaction(PCR),respectively.Results The CCK-8 assay showed that the cell survival rates were higher in the TGF-β1+melatonin,TGF-β1+melatonin+siDCN,and TGF-β1+DCN groups than the TGF-β1 group(P＜0.05).The cell sur-vival rate was higher for the TGF-β1+melatonin group than the TGF-β1+melatonin+siDCN group(P＜0.05).Western blotting and real-time PCR showed DCN and that E-cadherin were significantly down-regulated in the TGF-β1 group compared with the control group(P＜0.05).Compared with the TGF-β1 group,the TGF-β1+melatonin and TGF-β1+melatonin+siDCN groups showed up-regulated E-cadherin and DCN expression levels and down-regulated TGF-β1 and Smad2 expression levels(P＜0.05).Compared with the TGF-β1+melato-nin+siDCN group,the TGF-β1+melatonin+siDCN group showed up-regulated E-cadherin and DCN expression levels and down-regulated TGF-β1 and Smad2 expression levels(P＜0.05).Conclusion Melatonin can delay the EMT of human peritoneal mesoepithelial cells,and the DCNgene may be an important target to inhibit the EMT of these cells.关键词
褪黑素/人腹膜间皮细胞/核心蛋白聚糖/上皮-间质转化Key words
melatonin/human peritoneal mesothelial cell/decorin/epithelial-mesenchymal transdifferentiation分类
医药卫生引用本文复制引用
刘珊,张明霞,刘伦志..褪黑素对人腹膜间皮细胞上皮-间质转化的作用及机制[J].中国医科大学学报,2024,53(3):235-239,5.基金项目
湖北省教育厅科学研究计划(B2021155) （B2021155）
