中国比较医学杂志2024，Vol.34Issue(2)：16-24,9.DOI:10.3969/j.issn.1671-7856.2024.02.003
炎调方调控Rho/ROCK信号通路对脓毒症急性胃肠损伤小鼠肠上皮细胞凋亡的研究
Mechanism of Yantiao Fang regulating the Rho/ROCK signaling pathway in apoptosis of intestinal epithelial cells in mice with acute gastrointestinal injury caused by sepsis
摘要
Abstract
Objective To investigate the effect of Yantiao Fang on apoptosis of intestinal epithelial cells in mice with acute gastrointestinal injury caused by sepsis by regulating the Rho/ROCK signaling pathway.Methods Seventy BALB/c mice were randomly divided into normal,sham,and model groups.Except for normal and sham groups,mice were subjected to cecal ligation and perforation to establish a mouse model of acute gastrointestinal injury caused by sepsis.The mouse models were randomly divided into model,Low,Medium,and High dose of Yantiao Fang,and ROCK inhibitor groups.Histopathological changes of the ileum were observed by HE staining.Serum levels of IL-1β,IL-6,TNF-α,and IL-10 were measured by ELISA.PCNA and Ki-67 expression was detected by immunohistochemistry.Cleaved caspase3 and Bax expression was detected by Western blot.ROCK and MLC mRNA expression in the ileum was measured by RT-qPCR.Results Compared with normal and sham groups,Chiu's pathological score,proinflammatory factor(IL-1β,IL-6,and TNF-α)levels,cleaved caspase3 and Bax protein expression,and ROCK and MLC mRNA levels were increased in the model group(P＜0.05).Moreover,anti-inflammatory mediator IL-10 and expression of PCNA and Ki-67 in the ileum were decreased in the model group(P＜0.05).Compared with the model group,histopathological changes of the ileum in all Yantiao Fang groups were improved by various degrees with the increase in dose.Chiu's pathological score,IL-1β,IL-6,and TNF-α serum levels,cleaved caspase3 and Bax protein expression,and ROCK and MLC mRNA levels were decreased in Yantian Fang groups(P＜0.05).The IL-10 level and expression of PCNA and Ki-67 in the ileum were increased in Yantian Fang groups(P＜0.05).Conclusions Yantiao Fang may inhibit apoptosis of intestinal epithelial cells in mice with acute gastrointestinal injury due to sepsis by regulating the Rho/ROCK signaling pathway,thereby alleviating intestinal tissue inflammation and ultimately preventing intestinal mucosal tissue injury.关键词
脓毒症急性胃肠损伤/肠上皮细胞凋亡/Rho/ROCK信号通路/炎调方Key words
acute gastrointestinal injury caused by sepsis/apoptosis of intestinal epithelial cells/Rho/ROCK signaling pathway/Yantiao Fang分类
医药卫生引用本文复制引用
陈乾,王帝,张定一,贾赛蕾,王丽辉,曹玉净,李鲜,李燕红..炎调方调控Rho/ROCK信号通路对脓毒症急性胃肠损伤小鼠肠上皮细胞凋亡的研究[J].中国比较医学杂志,2024,34(2):16-24,9.基金项目
国家自然科学基金青年项目(82104833) （82104833）
全国名老中医药专家传承工作室(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
河南省中医药科学研究专项课题(2024ZY2084,20-21ZY2114,2022ZY1086) （2024ZY2084,20-21ZY2114,2022ZY1086）
河南省科技攻关项目(212102310339). （212102310339）
