中国甜菜糖业Issue(1)：21-24,4.DOI:10.3969/j.issn.1002-0551.2024.01.005
甜菜CEAP核心引物的筛选
Screening of Sugar Beet CEAP Core Primers
摘要
Abstract
In this paper,we aimed to select core primers with high polymorphism,strong stability and repetition from a CEAP primer not applied to sugar beet,and to find a suitable method for detecting CEAP primer polymor-phism.In this study,four CEAP molecular markers of cis elements,GATAA,TGAC,AAAG and ACGTG,closely related to plant growth development,signal transduction and stress response,were selected to amplify the genome of some beet varieties and screen 83 CEAP primer polymorphisms by using appropriate detection meth-ods.The results show that the optimal detection method is 2%agarose gel;17 primers from 83 CEAP primers,the polymorphism information content ranges from 0.2608 to 0-0.8446,the average is 0.6649;indicating that CEAP primers have high polymorphism,which can be used for the construction of beet fingerprint and genetic di-versity analysis.关键词
甜菜/CEAP引物/分子标记/核心引物Key words
sugar beet/CEAP primer/molecular marker/core primer分类
农业科学引用本文复制引用
宋玥,吴则东..甜菜CEAP核心引物的筛选[J].中国甜菜糖业,2024,(1):21-24,4.基金项目
财政部和农业农村部"国家现代农业产业技术体系(糖料)"项目(CARS-170111)资助. （糖料）
