中国甜菜糖业Issue(1)：29-34,6.DOI:10.3969/j.issn.1002-0551.2024.01.007
甘蔗木质素代谢途径关键酶生物信息学分析
Bioinformatics Study on Key Enzymes of Sugarcane Lignin Metabolism Pathway
摘要
Abstract
In order to understand the physicochemical properties of key enzymes of lignin metabolism pathway in sugarcrop-sugarcane,the sequence information of key enzymes(PAL,CAD,CCR,COMT)of lignin metabolism pathway was collected in the NCBI database,their physicochemical properties were analyzed by online software ExPASy-Prot Param,and the similarity of model plants and their sequences was compared by MEGA-7 soft-ware and an evolutionary tree was established.The results of their physicochemical properties showed that the key enzymes in the lignin metabolism pathway of sugarcane were mostly hydrophilic proteins,such as PAL,CCR,COMT of sugarcane,and CAD of sugarcane was hydrophobic protein.The key enzyme of the sugarcane lignin pathway is stability;The isoelectricpoints of the four key enzymes were neutral or weakly acidic;There lative mo-lecular mass and aminoacid number of PAL are different from those of other enzyme molecules.The results o f ev-olutionary tree sequences imilarity analysis showed that the CAD and COM molecules of sugarcane and sorghum were closely related,while thee volutionary relation ship of other molecules was farther.The PAL similarity of sug-arcane and switch grass is high;Sugarcane is closely related to wheat and corn.Through research,we can further understand the key enzymes of sugarcane lignin metabolism pathway,and provide some clues and references for future research on sugarcane lignin metabolism pathway.关键词
甘蔗/木质素代谢/关键酶/理化性质/进化树分析Key words
sugarcane/Lignin metabolism/Key enzymes/Physical and chemical properties/Evolutionary tree a-nalysis分类
农业科学引用本文复制引用
吴雪,刘乃新..甘蔗木质素代谢途径关键酶生物信息学分析[J].中国甜菜糖业,2024,(1):29-34,6.基金项目
黑龙江省省属高等学校基本科研业务费项目(2020-KYYWF-1028) （2020-KYYWF-1028）
黑龙江省博士后面上项目LBH-Z22245,纳米硒生物强化调控寒地香稻产量和品质的分子机制研究 （）
国家现代农业产业技术体系(糖料)建设项目"质量安全与营养品质评价"(CARS-170503). （糖料）
