circAGFG1对非小细胞肺癌生物学行为的影响机制研究OACSTPCD

Objective To investigate the mechanism of the effect of circular RNA(circRNA)AGFG1 on the biological behavior of non-small cell lung cancer(NSCLC)by targeting microRNA(miR)-374a-5p/vascular endothelial growth factor C(VEGFC)axis.Methods The expression levels of circAGFG1,miR-374a-5p and VEGFC mRNA in tumor tissues and adjacent tissues of 55 NSCLC patients,human bronchial epithelial cell line(BEAS-2B)and human NSCLC cell line(HCC827,H1975,A549,H1299)were detected by qRT-PCR.Taking A549 cells as the research object,control group,circAGFG1 small interfering RNA(si circAGFG1)group and negative control(si NC)group,si circAGFG1+miR-374a-5p inhibitor group,and si circAGFG1+inhibitor NC group were set up.The expression levels of circAGFG1,miR-374a-5p and VEGFC mRNA of A549 cells in each group were detected by qRT-PCR;CCK-8 and Transwell assays were used to detect the cell viability,migration and invasion abilities;Western blot was used to detect the expression of proliferating protein Ki-67,matrix metalloproteinase-9(MMP-9)and VEGFC;dual luciferase gene reporter assay was used to verify the targeting relationship between circAGFG1,VEGFC and miR-374a-5p,respectively.Results The expression levels of circAGFG1 and VEGFC mRNA in cancer tissues and human NSCLC cell lines were obviously increased,while the expression of miR-374a-5p was obviously decreased(P<0.05).Compared with the control group and the si NC group,the OD450 values after 24 hours and 48 hours of culture,migration number,invasion number,and the expression levels of Ki-67,MMP-9,circAGFG1,and VEGFC in the si circAGFG1 group were obviously decreased(P<0.05),while the expression of miR-374a-5p was obviously increased(P<0.05).Compared with the si circAGFG1+inhibitor NC group,the OD450 values after 24 hours and 48 hours of culture,migration number,invasion number,and the expression levels of Ki-67,MMP-9,and VEGFC in the circAGFG1+miR-374a-5p inhibitor group were obviously increased(P<0.05),the expression of miR-374a-5p was obviously decreased(P<0.05),while circAGFG1 had no obvious change(P>0.05).circAGFG1 and VEGFC had targeting relationships with miR-374a-5p,respectively.Conclusion Interference with the expression of circAGFG1 can inhibit the development of malignant biological behavior of A549 cells by regulating miR-374a-5p/VEGFC axis.