中国烟草学报2024，Vol.30Issue(2)：61-70,10.DOI:10.16472/j.chinatobacco.2023.T0123
普通烟草KCS基因家族的鉴定及非生物胁迫表达模式分析
Genome-wide Identification and Analysis of KCS Gene Family in Nicotiana tabacum under Abiotic Stress
摘要
Abstract
[Purpose]Beta-ketoacyl-CoA synthetase(KCS)is a key enzyme that regulates plant fatty acid synthesis and plays an important role in plant growth,development and in response to biological/abiotic stresses.However,the KCS gene in tobacco has not been reported yet.In order to investigate the role of the tobacco KCS gene under abiotic stress,this study conducted research on the tobacco KCS gene family.[Methods]Bioinformatics methods were employed in this study to analyze phylogenetic evolution,collinearity,and expression patterns of KCS genes.[Results]A total of 41 KCS genes were identified in the tobacco genome.The newly identified tobacco KCS genes and those from Arabidopsis thaliana could be divided into eight subgroups,most of which included members from both Arabidopsis thaliana and tobacco.A total of 10 NtKCS members originated from genome-wide replication events,and NtKCS10 and NtKCS11 are homologous to AtKCS02,suggesting they may perform similar biological functions.The NtKCS gene promoter contains numerous stress-related cis-elements,and their expression shows tissue specificity,with most members showing increased expression under drought and salt stress,suggesting NtKCS members may play a role in tobacco's stress response signal transduction.[Conclusion]In this study,the KCS gene family members in tobacco were systematically identified and analyzed.These results provide a theoretical basis for further exploring the biological functions of the tobacco KCS gene family.关键词
烟草/KCS基因/系统进化/表达模式/非生物胁迫Key words
tobacco/KCS,phylogenetic evolution/expression patterns/abiotic stresses引用本文复制引用
郭存,叶柯妤,孙晋浩,李东徽,貊志杰,李尊强,刘晓姗,李志远,李浩昊,段柯帆,郑吉云,田华,王瑗申,商暾..普通烟草KCS基因家族的鉴定及非生物胁迫表达模式分析[J].中国烟草学报,2024,30(2):61-70,10.基金项目
黑龙江省烟草行业科技开发项目"主栽品种龙江911蚜虫和PVY抗性分子标记辅助定向改良"(2022230000200048) （2022230000200048）
江苏中烟科技项目"低焦油烤烟新材料筛选及定向改良技术研究"(H202303) （H202303）
中央级公益性科研院所基本科研业务费专项(1610232023013) （1610232023013）
中国烟草总公司"揭榜挂帅"项目"中烟特香301品种品质保障技术优化及在中高档卷烟中的应用研究"(110202103015) （110202103015）
