中国烟草学报2024，Vol.30Issue(2)：125-134,10.DOI:10.16472/j.chinatobacco.2023.T0064
60Co-γ射线辐照对雪茄烟叶发酵微生物和代谢物变化及烟叶质量的影响
Effects of 60Co-γ ray irradiation on microorganisms,metabolites and quality of cigar tobacco during the fermentation
摘要
Abstract
[Purpose]We used 60Co-γ rays to sterilize bacteria,clarifying the significant role of microorganisms in cigar tobacco leaf fermentation process.[Method]Cigar tobacco leaves of the Dexue No.1 variety were fermented following 10k Gy of 60Co-γ ray irradiation.Microbial diversity,metabolomics,neutral aroma components,alkaloids,and other indices of cigar leaves were measured and analyzed during fermentation.[Results]1)No bacterial community was detected in irradiated cigar leaves fermented for 28 days,as determined by traditional isolation culture and Illumina MiSeq high-throughput sequencing.2)Non-targeted metabonomics showed higher levels of polyphenols,such as rutin,astragaloside,phloridzin,kaempferol,kaempferol-3-O-rutinoside,and dipeptides in irradiated tobacco leaves after 28 days of fermentation compared to non-irradiated leaves..Meanwhile,levels of amino acids like leucine,L-serine,and proline were lower..Metabolic pathways showing significant differences in KEGG enrichment included flavonoid and flavonol biosynthesis,and arachidonic acid metabolism.3)After 28 days of fermentation,significant reductions were observed in total alkaloids,reducing sugars,total sugars,starch,and protein content in both groups of tobacco leaves.The decrease in these indicators was less pronounced in irradiated leaves compared to non-irradiated leaves.After 28 days of fermentation,irradiated leaves had significantly lower neutral aroma components but higher browning reaction products,resulting in greater vitality,impurities,and irritability in cigar leaves.[Conclusion]A 10kGy dose of60Co-γ ray irradiation effectively sterilized the cigar leaves before fermentation.After 28 days of fermentation and transformation of macromolecules in irradiated leaves were lower than in non-irradiated leaves,leading to a reduced increase in sensory quality..Despite this,the quality of irradiated leaves still showed some improvement compared to non-fermented tobacco.关键词
雪茄烟叶/60Co-γ射线/发酵/微生物/代谢组学/烟叶质量Key words
cigar tobacco/60Co-γ ray/fermentation/microorganism/metabolomics/tobacco quality引用本文复制引用
张蓝月,秦艳青,赵园园,任梦娟,张瑞娜,史宏志..60Co-γ射线辐照对雪茄烟叶发酵微生物和代谢物变化及烟叶质量的影响[J].中国烟草学报,2024,30(2):125-134,10.基金项目
四川省烟草公司资助项目"优质雪茄烟叶发酵工艺优化及特色发酵技术开发"(SCYC202124) （SCYC202124）
