中国肿瘤生物治疗杂志2024，Vol.31Issue(3)：219-223,5.DOI:10.3872/j.issn.1007-385x.2024.03.002
白藜芦醇通过下调PRMT5表达抑制肝胆管癌SMMC-7721细胞的增殖、侵袭和细胞周期
Resveratrol inhibits proliferation,invasion and cell cycle of hepatobiliary carcinoma SMMC-7721 cells by downregulating the expression of PRMT5
摘要
Abstract
Objective:To investigate the effects of resveratrol(Res)on the proliferation,invasion,and cell cycle of hepatobiliary carcinoma SMMC-7721 cells by regulating protein arginine methyltransferase 5(PRMT5)expression and its underlying mechanism.Methods:Normal hepatocytes LO2 and hepatobiliary carcinoma SMMC-7721 cells were routinely cultured and treated with 20,40,80 μmol/L Res.qPCR assay was used to detect the mRNA expression of PRMT5 in LO2 cells,SMMC-7721 cells and Res-treated SMMC-7721 cells,respectively.The effects of Res on the proliferation,invasion,and cell cycle and apoptosis were examined using MTT assay,Transwell assay,and flow cytometry,respectively.WB assay was used to detect the protein expression of PRMT5,cyclin D1 and cyclin E1 in SMMC-7721 cells.Results:PRMT5 was highly expressed in SMMC-7721 cells(P<0.01).20,40,and 80 μmol/L Res significantly inhibited the mRNA and protein expression of PRMT5 in SMMC-7721 cells(all P<0.01),suppressed the proliferation(P<0.01)and invasion(P<0.05)abilities of SMMC-7721 cells,and blocked SMMC-7721 cell cycle in G2/M phase as well as promoted its apoptosis(all P<0.01);Besides,Res significantly inhibited the protein expression levels of cyclin D1 and cyclin E1 in SMMC-7721 cells(all P<0.01).Conclusion:PRMT5 is highly expressed in SMMC7721 cells.Res can effectively inhibit the proliferation and invasion abilities of SMMC-7721 cells and induce cell apoptosis,and its possible mechanism is related to the inhibition of PRMT5 expression.关键词
白藜芦醇/肝胆管癌/SMMC-7721细胞/蛋白质精氨酸甲基转移酶5/增殖/侵袭/细胞周期/凋亡Key words
resveratrol/hepatobiliary carcinoma/SMMC-7721 cell/protein arginine methyltransferase 5(PRMT5)/proliferation/invasion/cell cycle/apoptosis分类
临床医学引用本文复制引用
沈兴艳,孙象军..白藜芦醇通过下调PRMT5表达抑制肝胆管癌SMMC-7721细胞的增殖、侵袭和细胞周期[J].中国肿瘤生物治疗杂志,2024,31(3):219-223,5.基金项目
山东省重点研发计划(No.2108GSF118191) （No.2108GSF118191）
山东省医药卫生科技发展计划(No.2017WS321) （No.2017WS321）
