中国肿瘤生物治疗杂志2024，Vol.31Issue(3)：224-230,7.DOI:10.3872/j.issn.1007-385x.2024.03.003
虎杖苷通过Hippo/YAP通路影响甲状腺癌8505C细胞的恶性生物学行为和顺铂敏感性
Polydatin affects malignant biological behaviors and DDP chemosensitivity of thyroid cancer 8505C cells through the Hippo/YAP pathway
摘要
Abstract
Objective:To investigate the effect of polydatin on malignant biological behaviors and cisplatin(DDP)-sensitivity of human thyroid cancer 8505C cells by regulating the Hippo/Yes-associated protein(YAP)signaling pathway.Methods:8505C cells were cultured in vitro,and their DDP-resistant cells(8505C/DDP)were constructed.The proliferation ability of 8505C and 8505C/DDP cells that treated with 0,25,50,75,and 100 nmol/L polydatin was detected by CCK-8 assay,in order to screen the optimal action concentration of polydatin.The 8505C cells were divided into control group,polydatin group,empty vector group,polydatin+YAP1 overexpression group;and the 8505C/DDP cells were divided into control group,DDP group,DDP+polydatin group,DDP+empty vector group,and DDP+polydatin+YAP1 overexpression group.WB assay was used to detect the expression of Hippo/YAP pathway related proteins[YAP1,transcriptional coactivator factor(TAZ)]and EMT-associated proteins(E-cadherin,N-cadherin)in the 8505C cells of each group;Besides,the expression levels of YAP1,TAZ,and drug resistance-associated proteins[P-glycoprotein(P-gp),multidrug resistance-associated protein 1(MRP1)]as well as apoptosis-associated proteins(cleaved caspase-3,BAX,Bcl-2)in 8505C/DDP cells were also detected by WB.Transwell assay and cell scratching assay were used to detect the invasion and migration abilities of 8505C and 8505C/DDP cells in each group,respectively.Results:Polydatin significantly inhibited the proliferation of 8505C cells(P<0.05),but 8505C/DDP cells were resistant to low concentrations of polydatin(P<0.05).Moreover,polydatin significantly inhibited the protein expression of YAP1,TAZ and N-cadherin,elevated the protein expression of E-cadherin in 8505C cells(P<0.05),and significantly suppressed the migration and invasion of 8505C cells(all P<0.05);However,overexpression of YAP1 reversed the effects of polydatin on 8505C cells(all P<0.05).50 nmo/L polydatin significantly inhibited the protein expression of YAP1,TAZ,P-gp,MRP1 and Bcl-2,elevated the protein expression of cleaved caspase-3 and BAX in 8505C/DDP cells which treated with DDP(all P<0.05),and induced cell apoptosis(all P<0.05);However,overexpression of YAP1 reversed the effect of polydatin on 8505C/DDP cells(all P<0.05).Conclusion:Polydatin inhibits the malignant biological behaviors and enhances the DDP-sensitivity of 8505C cells via inhibiting the Hippo/YAP signaling pathway.关键词
甲状腺癌/8505C细胞/虎杖苷/Hippo/YAP通路/迁移/侵袭/上皮间质转化/化疗敏感性Key words
thyroid cancer/8505C cell/polydatin/Hippo/YAP pathway/migration/invasion/epithelial mesenchymal transition(EMT)/chemosensitivity分类
医药卫生引用本文复制引用
曹建中,黄金石,丁亚亭..虎杖苷通过Hippo/YAP通路影响甲状腺癌8505C细胞的恶性生物学行为和顺铂敏感性[J].中国肿瘤生物治疗杂志,2024,31(3):224-230,7.基金项目
南通市市级科技计划(No.JCZ20004) （No.JCZ20004）
