中国肿瘤生物治疗杂志2024，Vol.31Issue(3)：240-246,7.DOI:10.3872/j.issn.1007-385x.2024.03.005
雷公藤内酯醇通过调控miR-142-3p/HSP70通路抑制人乳腺癌MCF-7细胞增殖、侵袭和迁移
Triptolide inhibits proliferation,invasion and migration of human breast cancer MCF-7 cells by regulating miR-142-3p/HSP70 pathway
摘要
Abstract
Objective:To investigate the effects of triptolide(TP)on the malignant biological behaviors of human breast cancer MCF-7 cells through the miR-142-3p/HSP70 signaling pathway.Methods:MCF-7 cells were routinely cultured and divided into 6 groups:control group,TP group,miR-142-3p inhibitor group,TP+inhibitor group,miR-142-3p mimics group and TP+mimics group.The corresponding nucleic acids or plasmids were transfected into MCF-7 cells with transfection reagents.The mRNA expression of miR-142-3p and HSP70 in MCF-7 cells was detected by qPCR method.The proliferation,invasion and migration abilities of MCF-7 cells were detected by EdU cell proliferation assay,Transwell chamber assay,and cell scratch assay,respectively.The protein expression of HSP70 in MCF-7 cells was determined by WB assay.Results:TP or miR-142-3p overexpression significantly promoted the expression of miR-142-3p and HSP70 in MCF-7 cells,while knockdown of miR-142-3p significantly inhibited the expression of miR-142-3p and HSP70 in MCF-7 cells.TP could reverse the effects of miR-142-3p knockdown on the expression of miR-142-3p and HSP70 in MCF-7 cells.TP and miR-142-3p overexpression could significantly inhibit the proliferation,migration and invasion of MCF-7 cells(all P<0.05),while knockdown of miR-142-3p could promote the proliferation,migration and invasion of MCF-7 cells(all P<0.05).TP could reverse the effect of miR-142-3p knockdown on the malignant biological behavior of MCF-7 cells(all P<0.05).Conclusions:TP can inhibit the proliferation,invasion,and migration of MCF-7 cells by regulating the miR-142-3p/HSP70 signaling pathway.关键词
乳腺癌/雷公藤内酯醇/MCF-70细胞/增殖/侵袭/迁移/miR-142-3p/HSP70信号通路Key words
breast cancer/triptolide/MCF-7 cell/proliferation/invasion/migration/miR-142-3p/HSP70 signaling pathway分类
医药卫生引用本文复制引用
王进军,崔鹏来,程欣,钱梦悦,曾祥隽,徐子金,王怡帆..雷公藤内酯醇通过调控miR-142-3p/HSP70通路抑制人乳腺癌MCF-7细胞增殖、侵袭和迁移[J].中国肿瘤生物治疗杂志,2024,31(3):240-246,7.基金项目
武汉市卫健委科研基金(No.WX20Z16) （No.WX20Z16）
