茯苓酸通过调控AKT/MDM2/p53通路影响结直肠癌HCT116细胞的恶性生物学行为OA北大核心CSTPCD

Objective:To investigate whether pachymic acid(PA)affects the malignant biological behaviors of colorectal cancer(CRC)HCT116 cells through the AKT/MDM2/p53 pathway.Methods:HCT116 cells were routinely cultured and divided into control group,MK-2206(AKT inhibitor)group,low PA concentration(PA-L)group,high PA concentration(PA-H)group,and PA-H+SC79(AKT activator)group.CCK-8,Cell clone formation assay,flow cytometry and Transwell assay were used to detect the proliferation viability,clone formation ability,apoptosis,migration and invasion of HCT116 cells in each group.qPCR was used to determine the mRNA expression of E-cadherin,N-cadherin and vimentin,and WB assay was applied to detect the expression of AKT/MDM2/p53 pathway related proteins.Results:PA significantly suppressed the proliferation(P<0.05),clone formation ability,migration and invasion and induced apoptosis(all P<0.05)of HCT116 cells,inhibited the mRNA expression of N-cadherin and vimentin while promoted the mRNA expression of E-cadherin(all P<0.05),decreased the phosphorylation levels of AKT and MDM2(both P<0.05),and elevated the protein expression of p53(P<0.05)in HCT116 cells.The AKT inhibitor MK-2206 could mimic the effects of PA(all P<0.05),while its activator SC79 could reverse the effects of PA(all P<0.05).Conclusion:PA inhibits proliferation,migration and invasion and induces apoptosis of HCT116 cells by regulating the AKT/MDM2/p53 signaling pathway.