基于"脾开窍于口"探讨口腔微环境改变与大鼠胃幽门螺杆菌感染的相关性OACSTPCD

Objective:To explore the correlation between changes in oral microenvironment and helicobacter pylori infection in rats'stomach based on the concept of"spleen opening at the mouth".Methods:Thirty male SD rats were randomized into a blank group(n =10)and a model group(n =20).The blank group was given sterilized physiological saline by gavage,while the model group was gavaged by routine standard helicobacter pylori(Hp)solution,the oral mucosa of the rats in this group was impaired,and a small amount of bacterial solution was left in the mouth,once every other day,for 6 times.The successful colonization and modeling of Hp were confirmed using rapid urease detection and HE staining methods.Enzyme-linked immunosorbent assay(ELISA)was used to detect the expression of TLR4 and NLRP3 in serum,gastric mucosa,and oral mucosa,as well as the level of HP in oral mucosa and gastric mucosa.Person correlation analysis was employed to investigate the correlation of TLR4,NLRP3,and HP levels between in oral and gastric mucosa.Results:Rats infected with gastric Hp exhibited delayed activity,slow response,loose and rough hair,decreased appetite,decreased energy,and sometimes dry and sometimes loose stools.HE staining showed significant damage to the oral and gastric mucosa of the model group rats,and ELISA results showed that the expression levels of TLR4,NLRP3,and HP in the serum,oral mucosa,and gastric mucosa of the model group were significantly higher than those of the blank group(P<0.001).There is a positive correlation in the levels of TLR4,NLRP3,and HP between the oral mucosa and gastric mucosa(P<0.05).Conclusion:The alteration of oral microenvironment is an important factor leading to recurrent infection of helicobacter pylori,TLR4 and NLRP3 are upregulated during helicobacter pylori infection in rat stomach,and there is a positive correlation between the levels of TLR4,NLRP3,and HP in oral and gastric mucosa.