基于Fas/FasL介导的凋亡信号通路研究通管方对输卵管炎性不孕模型大鼠的作用机制OACSTPCD

Objective:To explore the effect of Tongguan formula(TGF)on the Fas/FasL pathway in the fallopian tube tissue of rats with salpingitis induced infertility(SI).Methods:Rat model of tubal inflammatory infertility was established by injecting mixed bacterial solution through the vagina.After modeling,77 SD female rats were randomly divided into a blank group,a sham operation group,a model group,a Fu Ke Jing(FKJ)capsule group(0.29 g/kg·d-1),a low dose group of TGF(7.515 g/kg·d-1),a medium dose group of TGF(15.03 g/kg·d-1),and a high dose group of TGF(30.06 g/kg·d-1).After 28 days of gavage administration,the rats in each group were euthanized and samples were taken.Protein immunoblotting(WB)was used to detect the expression levels of Fas,FasL protein,and Caspase-1 in the fallopian tube tissue of SI model rats.Enzyme-linked immunosorbent assay(ELISA)was used to detect the expression changes of IL-4,IL-10,and Caspase-1 in the serum of SI model rats.Results:Compared with the blank group,the levels of IL-4 and IL-10 in the serum of the model group were significantly reduced,while the level of Caspase-1 in the serum and fallopian tube tissue was significantly increased(P<0.01).Compared with the model group,the expression of Caspase-1 in serum and fallopian tube tissue was reduced in each dose group of TGF and the FKJ group(P<0.01),and the levels of IL-4 and IL-10 in serum,and the expression of Fas and FasL proteins in fallopian tube tissue were upregulated(P<0.05,P<0.01).Among them,the high-dose group of TGF had significantly better effects than other traditional Chinese medicine control groups and the FKJ capsule group(P<0.05).Conclusion:Tongguan formula may eliminate salpingitis in SI model rats by regulating the Fas/FasL apoptotic signaling pathway.