中医药学报2024，Vol.52Issue(4)：34-39,6.DOI:10.19664/j.cnki.1002-2392.240071
紫草素对过敏性紫癜性肾炎小鼠肾功能、炎症及p38 MAPK/NF-κB信号通路的影响
Effects of Shikonin on Renal Function,Inflammation and p38MAPK/NF-κB Signaling Pathway in Mice with Henoch-Schonlein Purpura Nephritis
摘要
Abstract
Objective:To investigate the effects of shikonin on renal function,inflammation and p38 mitogen-activated protein kinase(p38 MAPK)/nuclear factor-κB(NF-κB)signaling pathway in mice with Henoch-Schonlein purpura nephritis(HSPN).Methods:The HSPN mice model was established,and the successful mice were randomly divided into model group,cyclophosphamide group(22 mg/kg),shikonin low dose group(5 mg/kg)and shikonin high dose group(10 mg/kg),with 12 mice in each group.In addition,12 mice were left untreated and raised normally as the control group.Each group was given corresponding drug intervention for 28 days.Automatic biochemical analyzer and enzyme-linked immunosorbent assay were used to detect renal function and serum inflammation indexes respectively;HE staining was used to observe the histopathological changes of kidney;the messenger RNA(mRNA)and protein expressions of p38MAPK and NF-κB in kidney tissues were detected by quantitative PCR and Western blotting respectively.Results:In the control group,the renal tubules and glomeruli were clear and without inflammatory cell infiltration and mesangial hyperplasia.Compared with control group,the kidney tissues of model group mice showed severe inflammatory cell infiltration,severe mesangial hyperplasia,atrophy of renal tubules,blurred glomerular vascular network structure,vascular congestion and other pathological damage,and the serum creatinine(SCr),blood urea nitrogen(BUN),24 h urine protein(24 h UP),serum interleukin(IL)-6,IL-1β,tumor necrosis factor-α(TNF-α),p38MAPK and NF-κB mRNA and protein expression levels in kidney tissue were increased(P<0.05).Compared with model group,the inflammatory cell infiltration,vacuolar degeneration and swelling of renal tubular epithelial cells were improved in kidney tissues of cyclophosphamide group and shikonin low and high dose groups,and SCr,BUN,24 h UP,serum IL-6,IL-1β,TNF-α,p38MAPK and NF-κB mRNA and protein expression levels in kidney tissues were decreased(P<0.05);the improvement effect of above indexes in shikonin high dose group was better than that in shikonin low dose group(P<0.05).Conclusion:Shikonin can improve renal function and inflammatory response and reduce renal pathological injury in HSPN mice,and the mechanism may be related to the inhibition of p38MAPK/NF-κB signaling pathway activation.关键词
紫草素/过敏性紫癜性肾炎/p38丝裂原活化蛋白激酶/核因子-κB信号通路/肾功能Key words
Shikonin/Henoch-Schonlein purpura nephritis/p38 mitogen-activated protein kinase/nuclear factor-κB signaling pathway/Renal function分类
医药卫生引用本文复制引用
范颖,董娜,杨会慈,马靖,王云..紫草素对过敏性紫癜性肾炎小鼠肾功能、炎症及p38 MAPK/NF-κB信号通路的影响[J].中医药学报,2024,52(4):34-39,6.基金项目
河北省中医药类科研计划项目(2021285) （2021285）
