中山大学学报（医学科学版）2024，Vol.45Issue(2)：206-215,10.
纳米硒联合聚维酮碘对手术感染杀菌的效果
Bactericidal Effect of Selenium Nanoparticles Combined with Povidone-iodine on Pathogenic Bacteria in Surgical Site Infection
摘要
Abstract
[Objective]To investigate the bactericidal effect of loaded multifunctional povidoneiodine-nanometer sele-nium(PVP-I@Se)disinfectant on Staphylococcus aureus(SA)and methicillin-resistant Staphylococcus aureus(MRSA),and to provide an experimental basis for the reduction of surgical site infection(SSI).[Methods]The control group was the povidone iodine(PVP-I)group with different concentrations of iodine(50,75,100,200 and 400 μg/mL).The PVP-I@Se group(experimental group)was the PVP-I group further supplemented with 2 μg/mL Selenium nanoparticles(SeNPs).Then we compared the bactericidal effect of the two groups of disinfectant solutions on SA and MRSA by examin-ing the minimum inhibitory concentration(MIC),minimum bactericidal concentration(MBC),the shortest sterilization time at a concentration of 50 μg/mL iodine and the inhibition zone diameters at concentrations of 200 μg/mL and 400 μg/mL iodine.[Results]MIC values of PVP-I against SA and MRSA were both 79.17 μg/mL,and those of PVP-I@Se were 54.17 and 70.83 μg/mL,respectively.MBC values of PVP-I against SA and MRSA were 129.17 and 150.00 μg/mL,re-spectively,and those of PVP-I@Se were 70.83 and 87.50 μg/mL,respectively.At a concentration of 50 μg/mL iodine,the shortest sterilization time of PVP-I for SA and MRSA was 130 s and 140 s,respectively,and that of PVP-I@Se was 65 s and 75 s,respectively.At a concentration of 200 μg/ml iodine,the inhibition zone diameters of PVP-I for SA and MRSA were 7.67 mm and 8.33 mm,and those of PVP-I@Se were both 9.50 mm.At a concentration of 400 μg/mL iodine,the in-hibition zone diameters of PVP-I for SA and MRSA were 9.00 mm and 9.33 mm,and those of PVP-I@Se were 11.67 mm and 12.00 mm,respectively.[Conclusions]PVP-I with different concentrations of 50,75,100,200 and 400 μg/mL io-dine supplemented with 2 μg/mL SeNPs have better and faster bactericidal effect on SA and MRSA.When combined with SeNPs,PVP-I can enhance the bactericidal activity against SA and MRSA,but with better sensitizing effect on SA than MRSA and higher demand of iodine concentration(400 μg/mL)for sensitizing effect on MRSA.This study provides a theo-retical basis for selecting optimal concentration and action time of the disinfectant,thus reducing SSI.关键词
聚维酮碘/纳米硒/金黄色葡萄球菌/耐甲氧西林金黄色葡萄球菌/杀菌效果Key words
povidone iodine(PVP-I)/selenium nanoparticles(SeNPs)/staphylococcus aureus(SA)/methicillin resistant staphylococcus aureus(MRSA)/bactericidal effect分类
临床医学引用本文复制引用
何晓娟,罗学仕,陈金莺,余广超,钟敬祥,侯利环..纳米硒联合聚维酮碘对手术感染杀菌的效果[J].中山大学学报（医学科学版）,2024,45(2):206-215,10.基金项目
国家自然科学基金(81970806,82271094) （81970806,82271094）
广州市科技计划项目(202201020030,202201020015,202201010618) （202201020030,202201020015,202201010618）
广东省医学科研基金项目(A2022415) （A2022415）
