艾纳香油通过NF-κB非经典信号影响花生四烯酸代谢缓解LPS所致巨噬细胞的炎症反应OA北大核心CSTPCD

[Objective]To study the anti-inflammatory effects of Blumea balsamifera(L.)DC oil(BBO)based on nu-clear factor kappa-B(NF-κB)nonclassical and arachidonic acid(AA)pathway.[Methods]Effects of BBO on the produc-tion of slow reacting substance of anaphylaxis(SRS-A)were detected by the ileal smooth muscle method.The contents of prostaglandin E2(PGE2)and leukotriene B4(LTB4)in lipopolysaccharides(LPS)-induced macrophages were detected by ELISA kit.The expression of COX-2,5-LOX,FLAP and RelB were detected by qRT-PCR.Western blot was performed to detect the effects of BBO on the level of NF-κB nonclassical pathway proteins TNF receptor associated factor 3(TRAF3),TNF receptor associated factor 2(TRAF2),NF-κB-inducing kinase(NIK),p100 and RelB.[Results]The contractile tension of guinea pig ileum was reduced(P<0.001),and the SRS-A production inhibition rate reached 65.34%at 1mg·mL-1 BBO concentration.Compared with LPS group,BBO reduced the concentrations of PGE2(P<0.05)and LTB4(P<0.05),and decreased the expressions of COX-2(P<0.05),5-LOX(P<0.05)and FLAP(P<0.05)in AA pathway at con-centrations of 40-80 μg·mL-1.Moreover,40-80 μg·mL-1 BBO decreased the concentrations of TRAF3(P<0.05),TRAF2(P<0.05),and NIK(P<0.05),and further inhibited the phosphorylation of p100(P<0.05),as well as the level of the transcription factor RelB in genes(P<0.05)and proteins(P<0.05)in nonclassical NF-κB pathway,whereas BBO did not cause such changes.[Conclusion]BBO may potentially exert its anti-inflammatory effects by suppressing the regu-latory proteins TRAF3 and TRAF2 and the transcription factor RelB in NF-κB nonclassical pathway.The inhibitory action extending to the induction kinase function of NIK,further hindering the phosphorylation of p100 and its binding with the transcription factor RelB.Consequently,downstream elements in the AA pathway,including the pivotal rate-limiting en-zymes COX-2,5-LOX and FLAP,were altered.This modulation influences the levels of inflammatory mediators such as PGE2 and LTB4.