中山大学学报（医学科学版）2024，Vol.45Issue(2)：216-225,10.
艾纳香油通过NF-κB非经典信号影响花生四烯酸代谢缓解LPS所致巨噬细胞的炎症反应
Blumea balsamifera(L.)DC Oil Alleviates LPS-Induced Inflammatory Response in Macrophages by Affecting Arachidonic Acid Metabolism Via NF-κB Nonclassical Pathway
摘要
Abstract
[Objective]To study the anti-inflammatory effects of Blumea balsamifera(L.)DC oil(BBO)based on nu-clear factor kappa-B(NF-κB)nonclassical and arachidonic acid(AA)pathway.[Methods]Effects of BBO on the produc-tion of slow reacting substance of anaphylaxis(SRS-A)were detected by the ileal smooth muscle method.The contents of prostaglandin E2(PGE2)and leukotriene B4(LTB4)in lipopolysaccharides(LPS)-induced macrophages were detected by ELISA kit.The expression of COX-2,5-LOX,FLAP and RelB were detected by qRT-PCR.Western blot was performed to detect the effects of BBO on the level of NF-κB nonclassical pathway proteins TNF receptor associated factor 3(TRAF3),TNF receptor associated factor 2(TRAF2),NF-κB-inducing kinase(NIK),p100 and RelB.[Results]The contractile tension of guinea pig ileum was reduced(P<0.001),and the SRS-A production inhibition rate reached 65.34%at 1mg·mL-1 BBO concentration.Compared with LPS group,BBO reduced the concentrations of PGE2(P<0.05)and LTB4(P<0.05),and decreased the expressions of COX-2(P<0.05),5-LOX(P<0.05)and FLAP(P<0.05)in AA pathway at con-centrations of 40-80 μg·mL-1.Moreover,40-80 μg·mL-1 BBO decreased the concentrations of TRAF3(P<0.05),TRAF2(P<0.05),and NIK(P<0.05),and further inhibited the phosphorylation of p100(P<0.05),as well as the level of the transcription factor RelB in genes(P<0.05)and proteins(P<0.05)in nonclassical NF-κB pathway,whereas BBO did not cause such changes.[Conclusion]BBO may potentially exert its anti-inflammatory effects by suppressing the regu-latory proteins TRAF3 and TRAF2 and the transcription factor RelB in NF-κB nonclassical pathway.The inhibitory action extending to the induction kinase function of NIK,further hindering the phosphorylation of p100 and its binding with the transcription factor RelB.Consequently,downstream elements in the AA pathway,including the pivotal rate-limiting en-zymes COX-2,5-LOX and FLAP,were altered.This modulation influences the levels of inflammatory mediators such as PGE2 and LTB4.关键词
艾纳香油/脂多糖/NF-κB非经典通路/花生四烯酸代谢/抗炎作用Key words
Blumea balsamifera(L.)DC oil/lipopolysaccharides/nonclassical NF-κB pathway/arachidonic acid metabolism/anti-inflammatory effect分类
医药卫生引用本文复制引用
何贤芳,王万林,王鸿颖,刘瑞秀,易琼,王鲁..艾纳香油通过NF-κB非经典信号影响花生四烯酸代谢缓解LPS所致巨噬细胞的炎症反应[J].中山大学学报（医学科学版）,2024,45(2):216-225,10.基金项目
国家自然科学基金(32160849,31760747) （32160849,31760747）
