鼠李糖乳杆菌对老年小鼠术后海马区小胶质细胞激活及Tau蛋白磷酸化的影响OA北大核心CSTPCD

[Objective]To investigate the effects of Lactobacillus rhamnosus GG(LGG)on microglia and Tau phos-phorylation in the hippocampus of aged mice induced by anesthesia and surgery.[Methods]A total of thirty 18-month-old C57BL/6J mice were randomly divided into three groups:control group,anesthesia surgery group,and anesthesia surgery + LGG group(10 mice/group).The aged mice were oral administered by NS or LGG 109 CFU 150 μL once a day for 20 days.Then anesthesia surgery group and anesthesia surgery +LGG group received anesthesia with isoflurane and explorato-ry laparotomy.The activation status of microglia in the hippocampus was detected by immunofluorescence staining 12 hours after surgery.IL-6 concentration changes was detected by ELISA.The expression changes of Tau protein phosphorylation site(Tau-pS202/pT205)and total Tau protein was detected by western blot.[Results]The microglia in the hippocampus of the control group were in a resting state,and the concentration of inflammatory factor IL-6 was(82.08±12.07)pg/mL in control group.Compared to the control group,the anesthesia surgery group showed microglial cell Microglia were activat-ed,the concentration of inflammatory factors IL-6 increased significantly to(123.7±5.72)pg/mL(P=0.000),and the ex-pression of phosphorylated Tau-pS202/pT205 increased the hippocampus(P=0.002).Compared to the anesthesia surgery group,the activated microglia were inhibited,the concentration of IL-6 decreased to(96.68±9.59)pg/mL(P=0.008),and the expression of phosphorylated Tau-pS202/pT205 reduced significantly in the AS+LGG group(P=0.002).While there were no significant changes in total Tau protein among 3 groups.[Conclusion]Preoperative administration of probiot-ic LGG can alleviate the activation of microglia,increased secretion of inflammatory factors,and increased Tau protein phosphorylation levels in the hippocampus of elderly mice caused by anesthesia surgery.