雌性大鼠性早熟对糖脂代谢的影响OA北大核心CSTPCD

[Objective]To explore the effect of precocious puberty on glucose metabolism and lipid metabolism in fe-male rats.[Methods]Sixty two-day-old female rats were randomly divided into 2 groups.When aged 5 days,the preco-cious puberty group and normal group were given a single subcutaneous injection of danazol and solvent soybean oil respec-tively.The vaginal opening of rats was monitored from their 21 days of age.After 12 hours of fasting,all successful model-ing rats were randomly executed within 3 days after vaginal opening,when aged 7 and 12 weeks.Then we measured the rats'body weight and length,determined the concentrations of glucose,insulin,blood lipids,estradiol,leptin and adipo-nectin with enzyme-linked immunosorbent assay and observed the pathological changes of perirenal fat,uterus and ovary.[Results]For body weight and length,rats in the precocious puberty group were smaller than those in the normal group within 3 days after vaginal opening,but which did not affect their subsequent growth and development,and there was no significant difference between the two groups at 7 and 12 weeks of age.Within 3 days after vaginal opening,insulin levels had significant difference between the two groups(P = 0.001),the precocious group showed hyperinsulinemia and in-creased number of perirenal adipocytes.At three execution times,no significant difference was noted in estradiol,leptin and adiponectin levels between the two groups.The same was true in the ratios of ovary or uterus to body weight between the two groups.[Conclusions]Precocious puberty makes earlier onset of pubertal development and allows body maladapta-tion to the sudden changes of the internal environment.However,the changes due to precocious puberty are temporary and reversible,and they may become normal in adulthood.