吉林大学学报（医学版）2024，Vol.50Issue(2)：422-430,9.DOI:10.13481/j.1671-587X.20240215
呼吸道与非呼吸道标本分离MRSA临床株耐药性比较及其分子流行病学特征
Comparison of drug resistance of MRSA clinical strains from respiratory tract and non-respiratory tract specimens and their molecular epidemiological characteristics
摘要
Abstract
Objective:To discuss the differences in drug resistance,molecular typing,and biofilm-forming abilities of methicillin-resistant Staphylococcus aureus(MRSA)isolated from respiratory and non-respiratory tract specimens.Methods:A total of 100 MRSA strains were selected from clinical specimens of the hospitalized patients,and 50 MRSA strains were isolated from the respiratory specimens and another 50 MRSA strains were isolated from non-respiratory tract specimens.These 100 MRSA strains underwent an in vitro susceptibility test for 14 kinds of antimicrobial drugs,molecular typing was performed through multilocus sequence typing(MLST)and Staphylococcus aureus protein A(Spa)typing,and their biofilm-forming abilities were detected by crystal violet staining assay.Results:The in vitro susceptibility tests results showed that all the 100 MRSA strains had high overall resistance rates of over 60.0%to penicillin,erythromycin,clindamycin,levofloxacin,ciprofloxacin,moxifloxacin,and tetracycline,while only 9.0%exhibited resistance to trimethoprim-sulfamethoxazole,and all the strains were susceptible to vancomycin,linezolid,tigecycline,and quinupristin/dalfopristin.Compared with those isolated from non-respiratory tract specimens,the MRSA strains isolated from respiratory tract specimens showed significantly higher resistance rates to moxifloxacin,levofloxacin,ciprofloxacin,tetracycline,and gentamicin(P<0.05).Among the 100 MRSA strains,17 genotypes were identified,and the dominant types weve ST5-t2460(26.0%),ST239-t030(23.0%),and ST59-t437(20.0%).In the MRSA from the respiratory tract specimens,the dominant types were ST5-t2460(20.0%)and ST239-t030(13.0%),followed by ST59-t437(7.0%);in the non-respiratory tract specimens,13 genotypes were found,and the dominant types were ST59-t437(13.0%)and ST239-t030(10.0%).All 100 MRSA strains were biofilm producers,among them the strong biofilm formers,moderate biofilm formers,and weak biofilm formers accounted for 2.0%(2/100),24.0%(24/100),and 74.0%(74/100),respectively.The ST59-t437 clones had overall strong biofilm-forming abilities,and 60.0%(12/20)stains were moderate to strong biofilm formers.Conclusion:The MRSA strains isolated from the respiratory tract specimens have a significantly higher overall resistance rate than those isolated from the non-respiratory tract specimens.The ST59-t437 and ST239-t030 genotypes are dominant clones common to both types of specimens,and ST59-t437 strains show strong biofilm-forming abilities,and ST239-t030 strains exhibit the strongest resistance.关键词
耐甲氧西林金黄色葡萄球菌/耐药性/分子分型/生物膜Key words
Methicillin-resistant Staphylococcus aureus/Drug resistance/Molecular typing/Biofilm分类
医药卫生引用本文复制引用
额尔德木图,王艳艳,李喻瞳,陈贵林,王俊瑞..呼吸道与非呼吸道标本分离MRSA临床株耐药性比较及其分子流行病学特征[J].吉林大学学报（医学版）,2024,50(2):422-430,9.基金项目
国家自然科学基金项目(81660352) （81660352）
内蒙古自治区科技厅自然科学基金面上项目(2020MS08110) （2020MS08110）
内蒙古医科大学大学生科技创新"英才培育"项目(YCPY2021068) （YCPY2021068）
内蒙古医科大学大学生创新创业训练计划项目(202110132038) （202110132038）
