吉林大学学报（医学版）2024，Vol.50Issue(2)：442-450,9.DOI:10.13481/j.1671-587X.20240217
缺氧对宫颈癌SiHa细胞中Gli1与Sox2表达的影响
Effect of hypoxia on expressions of Gli1 and Sox2 in cervical cancer SiHa cells
摘要
Abstract
Objective:To analyze the expressions of glioma-associated oncogene homolog 1(Gli1)and sex determining region Y 2(Sox2)in the cervical cancer cells and their pathological significances,and to discuss the correlation between the expression of Gli1 and the stemness characteristics of the cervical cancer.Methods:Immunohistochemical staining was used to detect the expression of Gli1,hypoxia-inducible factor 1-alpha(HIF-1α),Sox2,sex determining region Y 9(Sox9),cluster of differentiation 44(CD44),octamer-binding transcription factor 4(OCT4),and lysine-specific demethylase 1(LSD1)in 159 cases of cervical cancer tissues and their correlations were analyzed.Gene Expression Profiling Interactive Analysis(GEPIA)was used to clarify the effect of Gli1 expression on the prognosis of the patients with cervical carcinoma and its correlation with the stem cell markers.The sphere-forming ability and the expression of stem cell markers in the SiHa cells under hypoxic status were detected.Results:The immunohistochemical staining results showed that the expression rate of Gli1 in cervical cancer tissue was positively correlated with the expression level of HIF-1α(r=0.374,P<0.001)and the expression level of Sox2(r=0.176,P<0.05).The GEPIA Database analysis results showed that the cervical cancer patients with positive Gli1 expression had poor prognosis(P<0.05).Under hypoxic status,the expression levels of Gli1,HIF-1α,and Sox2 mRNA and proteins in the SiHa cells were significantly increased(P<0.001),and the cells exhibited a strong sphere-formation ability.Conclusion:Hypoxia upregulates the expression levels of Gli1 and Sox2 mRNA and protein in the cervical cancer cells,and promotes the formation of stemness characteristics of the cancer cells.关键词
宫颈肿瘤/胶质瘤相关癌基因同源物1/缺氧/干性/缺氧诱导因子1aKey words
Cervical neoplasm/Glioma-associated oncogene homolog 1/Hypoxia/Stemness/Hypoxia-inducible factor 1-alpha分类
医药卫生引用本文复制引用
朱长吉,刘兴哲,玄延花..缺氧对宫颈癌SiHa细胞中Gli1与Sox2表达的影响[J].吉林大学学报（医学版）,2024,50(2):442-450,9.基金项目
国家自然科学基金项目(82160594) （82160594）
