重庆医学2024，Vol.53Issue(7)：998-1003,6.DOI:10.3969/j.issn.1671-8348.2024.07.007
粪便差异菌群、代谢物联合弥散张量成像对早产儿脑白质损伤的诊断价值
Diagnostic value of fecal differential flora and metabolites combined with diffusion tension imaging in premature infants with white matter injury
摘要
Abstract
Objective To explore the diagnostic value of fecal 16S rRNA gene sequencing,metabolomics technology combined with diffusion tension imaging(DTI)in premature infants with white matter injury(WMI).Methods A total of 71 premature infants with gestational age＜32 weeks and body weight＜1 500 g treated in this hospital from April to December 2022 were selected as the research subjects and divided into the WMI group(n=23)and non-WMI group(n=48)according to the MRI results.The fecal samples were col-lected within 24 h after birth,on 14,28 d after birth,and 16S rRNA gene sequencing and LC-MS/MS metabo-lomics analysis were performed.All subjects underwent brain MRI+DTI examination when corrected gesta-tional age was 37-40 weeks.The diagnostic efficiency of the combination of intestinal flora structure,differ-ential metabolites and DTI on WMI was studied by receiver operator characteristic curve(ROC).Results The area under the curve(AUC)for diagnosing WMI based on the generic level was 0.88(95％CI:0.74-＜1.00).AUC of WMI1 and non-WMI1 differential metabolite set for diagnosing WMI was 0.98(95％CI:0.96-0.99).The ADC value of the splenium of corpus callosum(r2=0.230 2,P=0.001)and FA value of occipital white matter(r2=0.291 0,P=0.001)were most related to intestinal flora structure.AUC of differential flo-ra and metabolites combined with FA values(lateral ventricle white matter and corpus callosum pressure)in the diagnosis of WMI was 0.98(95％CI:0.93-＜1.00).Conclusion Characteristic intestinal flora,differen-tial metabolites and DTI parameters could distinguish WMI from non-WMI to a great extent.关键词
早产儿脑白质损伤/肠道菌群/16S rRNA基因测序/LC-MS/MS代谢组学分析/弥散张力成像Key words
white matter injury of preterm infant/intestinal flora/16S RNA sequencing/metabolomics analysis of LC-MS/MS/diffusion tension imaging分类
医药卫生引用本文复制引用
刘玲,罗晗,李银珊,李承燕,敖当,林少珠..粪便差异菌群、代谢物联合弥散张量成像对早产儿脑白质损伤的诊断价值[J].重庆医学,2024,53(7):998-1003,6.基金项目
广东省湛江市科技计划项目(2021A05078) （2021A05078）
广东医科大学附属医院临床研究项目(LCYJ2020DL01,LCYJ2019B009). （LCYJ2020DL01,LCYJ2019B009）
