白细胞介素17及白细胞介素1β在成人喉乳头状瘤疾病进展中的相关性研究OACSTPCD

OBJECTIVE To explore relationship between the progression of adult laryngeal papilloma disease and HPV infection status with IL-17 and IL-1β.METHODS Tissue samples were collected from adult patients with laryngeal papilloma who underwent surgical treatment at the Department of Otolaryngology Head and Neck of Beijing Tongren Hospital from January 2010 to December 2020.We divide patients into papilloma group and disease progression group.RNA in situ hybridization was performed to determine HPV typing,and immunofluorescence was used to detect IL-17 and IL-1β expression,analysis the differences in IL-17 and IL-1β expression and correlation with high-risk HPV type.RESULTS There were significant differences in the positivity rates of IL-17 and IL-1β immunofluorescence between the papilloma group and the disease progression group.The positivity rates of IL-17 and IL-1β were significantly increased in the disease progression group(t=5.460,P<0.001;t=10.030,P<0.001).The high-risk type of HPV is correlated with the expression of IL-17 and IL-1β.The expression levels of IL-17 and IL-1β are significantly increased in HPV high-risk type patients,and the difference in IL-17 expression is statistically significant(t=4.554,P=0.0004).CONCLUSION There is a close correlation between the progression of adult laryngeal papilloma disease and IL-17 and IL-1β.High risk HPV exacerbates local expression of IL-17 and IL-1β in tissues,then causes changes in the local immune microenvironment and leading to disease progression.