中国耳鼻咽喉头颈外科2024，Vol.31Issue(3)：147-151,5.DOI:10.16066/j.1672-7002.2024.03.003
外源性白细胞介素10抑制嗜酸性粒细胞型慢性鼻窦炎伴鼻息肉中白细胞介素33表达
Exogenous IL-10 inhibits the expression of IL-33 in eosinophilic chronic sinusitis with nasal polyps
摘要
Abstract
OBJECTIVE To investigate the expression levels of interleukin-10(IL-10)and IL-33 in patients with chronic rhinosinusitis with nasal polyps(CRSwNP)and the effect of exogenous IL-10 stimulation on the expression level of IL-33 in eosinophilic CRSwNP(ECRSwNP).METHODS The expression levels of IL-10 and IL-33 in nasal polyp stissues of 12 patients in ECRSwNP group,8 patients in non-eosinophilic CRSwNP(nECRSwNP)group,and normal nasal mucosa of 12 healthy controls were determined by ELISA.Another 10 ECRSwNP samples were collected and cultured in medium with or without 100 ng/ml IL-10 for 24 hours,and then the expression of IL-33 was detected by ELISA and immunohistochemistry.RESULTS There was no significant difference in expression of IL-10 among ECRSwNP group,nECRSwNP group and healthy control group(P>0.05).The expression level of IL-33 in ECRSwNP group was higher than that in healthy control group,and the difference is statistically significant(P=0.0043).The expression level of IL-10 and IL-33 was a significant negative correlation in ECRSwNP group(r=-0.7470,P=0.0052).There was no significant correlation in nECRSwNP group and control group(P>0.05).Compared with the control group,the expression level of IL-33 in the IL-10-stimulated ECRSwNP group was significantly decreased(P=0.0197),and a significant decrease of IL-33 positive cells was observed in immunohistochemistry(P=0.0183).CONCLUSION IL-33 expression is significantly increased in ECRSwNP,and IL-10 is negatively correlated with IL-33.Exogenous IL-10 can inhibit IL-33 expression in ECRSwNP.关键词
鼻窦炎/鼻息肉/嗜酸细胞/白细胞介素10/白介素-33/嗜酸性粒细胞型慢性鼻窦炎伴鼻息肉Key words
Sinusitis/Nasal Polyps/Eosinophils/Interleukin-10/Interleukin-33/eosinophilic chronic rhinosinusitis with nasal polyps引用本文复制引用
赵亚娟,玄丽佳,谢希然,王向东,张罗..外源性白细胞介素10抑制嗜酸性粒细胞型慢性鼻窦炎伴鼻息肉中白细胞介素33表达[J].中国耳鼻咽喉头颈外科,2024,31(3):147-151,5.基金项目
国家重点研发计划(2022YFC2504100) （2022YFC2504100）
教育部长江学者创新团队(IRT13082) （IRT13082）
国家自然科学基金(82171110、82101189) （82171110、82101189）
首都卫生发展科研专项重点攻关项目(2022-1-1091) （2022-1-1091）
北京市自然科学基金(7222024) （7222024）
科技新星交叉合作项目(20220484226、20230484476) （20220484226、20230484476）
北京市卫生系统高层次公共卫生人才建设项目领军人才(01-08、02-09) （01-08、02-09）
