甘肃医药2024，Vol.43Issue(3)：193-197,212,6.
超声辅助酶法优化鸡骨草多糖提取工艺与抗炎研究
Studies on the ultrasound-assisted enzymatic extraction and anti-inflammatory activity of abrus cantoniensis hance polysac-charide
摘要
Abstract
Objecitve:On the basis of single factor experiment,response surface experiment was performed to optimize the extraction process of polysaccharide from Abrus cantoniensis Hance by ultrasound-assisted enzyme method.Its anti-inflammatory activity was analyzed by anti-inflammatory test in vitro.Methods:The effects of enzyme dosage,liquid-solid ratio,pH of enzymolysis,enzymolysis-ultrasonic power,enzymolysis-ultrasonic time,enzymolysis-ultrasonic temperature on the extraction rate of polysaccharide were investigated by single factor test.The extraction process of polysaccharide was optimized by response surface test.The contents of TNF-α,IL-1β,IL-6 and IL-10 were detected by ELISA method.Results:The optimized extraction conditions of Abrus cantoniensis Hance polysaccharide were as follows:cellulase content 2.8%,liquid-solid ratio 21∶1(mL/g),pH 5.0,enzymolysis-ultrasonic power 250 W,enzymolysis-ultrasonic temperature 49℃,enzymolysis-ultrasonic time 90 min.Under these conditions,the extraction rate of polysaccharide was 13.26%.Compared with the LPS group,the polysaccharide could decrease the contents of pro-inflammatory cytokines TNF-α,IL-1β and IL-6,and increase the contents of anti-inflammatory cytokine IL-10.Conclusion:The extraction efficiency of Abrus cantoniensis Hance polysaccharide by ultrasound-assisted enzyme method was high,and the extracted polysaccharide possessed strong anti-inflammatory activity.关键词
鸡骨草多糖/超声辅助酶法/响应面法/抗炎Key words
polysaccharides from Abrus cantoniensis Hance/ultrasound-assisted enzymatic method/response surface method/anti-inflammation分类
医药卫生引用本文复制引用
戴颖,戴薇,李翔,宁青..超声辅助酶法优化鸡骨草多糖提取工艺与抗炎研究[J].甘肃医药,2024,43(3):193-197,212,6.基金项目
2023年全国中药特色技术传承人才培训项目[编号:国中医药人教函(2023)96号] （2023）
