响应面法优化桑叶黄酮提取工艺及其抗氧化活性研究OA

The objective of this study was to optimize the extraction process of flavonoids from mulberry leaves and explore its antioxidant activity.The experiment selected microwave power,mi-crowave time,ethanol concentration,solid-liquid ratio and extraction time as variables,the yield of flavonoids as the evaluation index.Single factor tests and Box-Behnken method were used to opti-mize the extraction process.The scavenging ability on 1,1-diphenyl-2-picrylhydrazyl(DPPH·),hy-droxyl radical(·OH)and superoxide(O2-·)free radicals of flavonoids from mulberry leaves were also tested to estimate the antioxidant activities in vitro.The results showed that:optimal extraction rate(11.393±0.049 mg/g highest yield)could be achieved under the conditions of microwave power 175 W,microwave time 4.5 min,ethanol concentration 48.5%,solid-liquid ratio 30 mL/g.The abi-lity of mulberry leaves flavonoids extract to scavenge DPPH·,·OH and O2-·was great and scavenge ability was positively correlated with concentration of the extract.To sum up,microwave extraction technology can effectively extract of mulberry leaves flavonoids,leading to better antioxidant acti-vity.