广西师范大学学报（自然科学版）2024，Vol.42Issue(2)：200-215,16.DOI:10.16088/j.issn.1001-6600.2023041406
植物生长期根部病害生防菌筛选方法改进及其装置研制
Improvement of Screening Method and Device Development of Biocontrol Bacteria for Plant Root Diseases During Growth Period
摘要
Abstract
The current indoor screening methods for biocontrol bacteria tend to ignore the basic role of plants;to overcome this drawback,this paper compares the methods of indoor screening for biocontrol bacteria of dual culture,liquid medium screening and seedling substrate screening,then develops a new device based on the improved seedling substrate screening method.This device was modified of the PP-500 mL tissue culture bottle which resistant to high temperature(135 ℃)and high pressure(0.15 MPa).The columnar space of the tissue culture bottle was divided into 3 zones,there were plant growing zone,plant pathogen culture zone,and the candidate biocontrol strain culture one.Each zone was separated by a 0.45 pm nitrate fiber filter membrane.The plant growing zone was open,but the plant pathogen and the candidate biocontrol strain culture zones were closed with a lid.In this device,root exudates of plant growing in approximation of natural conditions and soil exudates were used as substrates to screen candidate biocontrol bacteria,so the results obtained were more reliable.The antagonistic effect of pathogen and candidate biocontrol bacteria was reflected by the change of cell density in this device,which was easy to detect and the result was definite.The device had the advantages of simple structure,low cost and convenient operation.It can be used for batch screening of candidate biocontrol bacteria against various plant root pathogens,but not suitable for screening of that against pathogens of leaf and stalk.关键词
病原菌/生防菌/筛选/铜绿假单胞菌/拮抗Key words
pathogenic fungi/biocontrol bacteria/screening/Pseudomonas aeruginosa/antagonism分类
农业科技引用本文复制引用
黄植清,刘莹儿,黄名丽,陈英绘,尚常花,陆祖军..植物生长期根部病害生防菌筛选方法改进及其装置研制[J].广西师范大学学报（自然科学版）,2024,42(2):200-215,16.基金项目
广西重点研发计划项目(2021AB27009) （2021AB27009）
