广州化学2024，Vol.49Issue(2)：73-77,5.DOI:10.16560/j.cnki.gzhx.20240217
纳米金-钴离子比色探针的制备及对半胱氨酸的测定
Preparation of Nanometer Gold-cobalt Ion Colorimrtric Probe and Determination of Cysteine
摘要
Abstract
In this paper,ascorbic acid was used to reduce chlorauric acid to prepare gold nanoparticles.In the presence of cobalt ion,cysteine induced gold nanoparticles to agglomerate and the color of the solution changed from red to blue.A new method for determination of cysteine was established.The preparation conditions of gold-cobalt ion colorimetric probe were discussed and the experimental conditions for determination of cysteine were investigated.The results show that:The optimal reaction time and pH of buffer solution were 20 min and 3.0 respectively.The linear regression equation for the determination of cysteine was A725/A520 =0.3965C+0.0133(C:μM),R2=0.9882.The linear range was 1×10-7 mol/L to 1×10-6 mol/L,and the detection limit was 2.2×10-8 mol/L.The recovery rate of urine samples from healthy people was determined by standard addition method,and the recovery rate was 92.8%～95.8%.A simple method for the determination of cysteine was developed.关键词
纳米金/钴离子/比色法/半胱氨酸/测定Key words
nanometer gold/cobalt ion/colorimetry/cysteine/determination分类
化学引用本文复制引用
徐永智,毛丽惠,刘雯雯,卜欣欣,徐康远,张伟..纳米金-钴离子比色探针的制备及对半胱氨酸的测定[J].广州化学,2024,49(2):73-77,5.基金项目
2022年度信阳学院大学生科研项目(2022-DXSLYB-009) （2022-DXSLYB-009）
