河南城建学院学报2024，Vol.33Issue(2)：122-128,7.DOI:10.14140/j.cnki.hncjxb.2024.02.018
超声辅助纤维素酶法提取小麦硒多糖工艺优化及抗氧化活性研究
Optimization of ultrasound-assisted cellulase extraction of selenium polysaccharide from wheat and its antioxidant activity
景磊 1王婷婷 1胡晨曦 1程美蝶1
作者信息
- 1. 河南城建学院 生命科学与工程学院,河南 平顶山 467036
- 折叠
摘要
Abstract
Selenium polysaccharides were extracted from selenium-rich whole wheat flour using ultrasound-as-sisted cellulase method.The extraction process of wheat selenium polysaccharides was optimized by single-fac-tor experiment and response surface methodology,with the extraction rate of selenium polysaccharides as the e-valuation index.The antioxidant activity of wheat selenium polysaccharides against ABTS+ was also studied.The results showed that the optimal extraction conditions for wheat selenium polysaccharides were a solid-liquid ratio of 1:17(g:mL),cellulase concentration of 0.75%,ultrasound temperature of 60℃,ultrasound power of 497 W,and ultrasound time of 50 minutes.Under these conditions,the extraction rate of wheat selenium pol-ysaccharides was38.24%,and the selenium content was 0.093 mg/kg.The vitro antioxidant activity study showed that the IC50 value of wheat selenium polysaccharides for ABTS+ radical scavenging was4.56 mg/mL.关键词
小麦硒多糖/超声辅助纤维素酶法/响应面法优化/抗氧化活性Key words
selenium polysaccharide from wheat/ultrasonic-assisted cellulase/response surface method optimi-zation/anti-oxidant activity分类
化学化工引用本文复制引用
景磊,王婷婷,胡晨曦,程美蝶..超声辅助纤维素酶法提取小麦硒多糖工艺优化及抗氧化活性研究[J].河南城建学院学报,2024,33(2):122-128,7.
