华南农业大学学报2024，Vol.45Issue(3)：311-320,10.DOI:10.7671/j.issn.1001-411X.202304045
Pcgf2促进鸡胚成纤维细胞重编程形成诱导多能干细胞
Pcgf2 promotes the reprogramming of chicken embryonic fibroblasts into induced pluripotent stem cells
摘要
Abstract
[Objective]We aimed to improve the stemness of chicken induced pluripotent stem cells using the Pcgf2 gene together with conventional reprogramming factors to induce chicken pluripotent stem cells.[Method]Six conventional reprogramming factors including Oct4,Sox2,Nanog,Lin28,C-myc and Klf4 as well as Pcgf2 gene were amplified and reconstituted with lentiviral vectors as lentiviral plasmids using chicken early embryos as templates.After packaging the lentivirus and measuring the corresponding viral titers,chicken embryonic fibroblasts were infected in groups and stem cell markers were assayed during the induction process.[Result]The cells induced by the combination of conventional reprogramming factors and Pcgf2 all expressed phosphatase activity early.Inducing the pluripotent stem cells with Pcgf2 could make the cells appear stem-like morphology earlier,and the expressions of endogenous genes Oct4,Sox2,Nanog and Lin28 increased more stably.[Conclusion]Pcgf2 can effectively enhance the reprogramming of cells to approach the stem cell state after being added to the induction system.The research provides a theoretical reference for the selection of reprogramming factors to induce complete reprogramming of cells in chickens and the mechanism study.关键词
鸡/慢病毒/多能转录因子/鸡胚成纤维细胞/体细胞重编程/诱导多能干细胞Key words
Chicken/Lentivirus/Pluripotent transcription factor/Chicken embryonic fibroblast/Somatic cell reprogramming/Induced pluripotent stem cell分类
农业科技引用本文复制引用
姚梓淇,廖立钦,宋佳蓓,张新珩,谢青梅..Pcgf2促进鸡胚成纤维细胞重编程形成诱导多能干细胞[J].华南农业大学学报,2024,45(3):311-320,10.基金项目
岭南现代农业科学与技术广东省实验室河源分中心双聘团队项目(DT20220003) （DT20220003）
广东省科技计划(2020B1212060060) （2020B1212060060）
广东省农业产业技术体系首席专家项目(2022KJ128,2023KJ128) （2022KJ128,2023KJ128）
广东省基础与应用基础研究基金(2023A1515010584) （2023A1515010584）
