秃杉素对水绵的抑制活性及叶绿体超微结构的影响OA北大核心CSTPCD

[Objective]The inhibition activity of 4-O-α-thevetopyranosyldiphyllin(TSC-3),extracted from Taiwania flousiana,on Spirogyra communis and its effect on S.communis chloroplast were investigated.[Method]The IC50 of 4-O-α-thevetopyranosyldiphyllin on chlorophyll and carotenoids in S.communis were determined by the ethanol extraction-spectrophotometric method.The effects of 4-O-α-thevetopyranosyldiphyllin on the microstructure and ultrastructure of S.communis were observed using microscopy and transmission electron microscopy.[Result]The IC50 of 4-O-α-thevetopyranosyldiphyllin on the contents of carotenoids,chlorophyll a,chlorophyll b and total pigments of S.communis after 7 d treatment were 3.41,31.50,47.34 and 29.30 mg·L-1,respectively.Further investigation showed that 4-O-α-thevetopyranosyldiphyllin could destroy the cells of S.communis,inducing broken cell wall,disappeared cell membrane,disintegrated chloroplast,completely disappeared thylakoids,dispersed starch granules,degraded and fragmented pyrenoids.[Conclusion]4-O-α-thevetopyranosyldiphyllin can reduce the contents of chlorophyll a,chlorophyll b and carotenoids in S.communis.The inhibition rate of carotenoids was higher than those of the other pigments.4-O-α-thevetopyranosyldiphyllin mainly acts on the chloroplasts and cell walls.This study provides a basis for further study of the molecular herbicidal mechanism of the plant-derived herbicidal chemical 4-O-α-thevetopyranosyldiphyllin,and also provides a theoretical basis for the control of aquatic algae.