核农学报2024，Vol.38Issue(6)：1035-1047,13.DOI:10.11869/j.issn.1000-8551.2024.06.1035
玉米ECT家族成员的全基因组分析及非生物胁迫下的响应表达
Genome-Wide Analysis of Maize ECT Family Members and Their Expressions in Response to Abiotic Stresses
摘要
Abstract
ECT domain protein family,an important post-transcriptional regulator,participates in the regulation of m6A modification,and plays an important role in regulating the gene expression level of plants under normal and stress conditions and maintaining their normal growth and development.To investigate the function of ECT domain protein family in the growth and development of maize(Zea mays L.)under abiotic stress and stress response,in this study,22 members of maize ECT family were identified by bioinformatics,and their sequences and structural characteristics,chromosome distribution,promoter cis-acting elements,GO enrichment,protein-protein interaction networks and phylogenetic evolution were analyzed.The expression patterns of maize ZmECTs were analyzed by quantitative real-time PCR(qRT-PCR)under cold stress and different hormone treatments.Result showed that the members of maize ECT family were distributed on 10 chromosomes,the encoded proteins ranged from 119 to 748 aa,the relative molecular weight(MW)ranged from 13 412.17 to 81 823.70 Da,and the isoelectric point(pI)ranged from 5.43 to 8.82.Most of the proteins are localized in the nucleus.10 motifs were identified in ZmECT family.The analysis of cis-acting elements showed that the promoters of ZmECT family members contained multiple response elements related to stresses,hormones,growth and development.GO enrichment analysis showed that ZmECT family members may participate in mRNA splicing and maintaining RNA stability.Protein-protein interaction networks predicted that ZmECT6 was the core member of this family proteins.The phylogenetic tree showed that the ZmECT family members were divided into 4 groups.qRT-PCR results showed that ZmECT family members presented complex response patterns after different hormone treatments.In addition,some ZmECT family members could respond to cold stress.The above results can provide reference for the subsequent functional analysis of maize ECT genes and the study of abiotic stress,and also provide available gene resources for breeding excellent resistant maize varieties.关键词
玉米(Zea mays L.)/ECT家族/生物信息学/非生物胁迫/响应表达Key words
maize(Zea mays L.)/ECT gene family/bioinformatics/abiotic stress/response expression引用本文复制引用
徐可丽,周美君,郭怡婷,吉虹菁,黄敏,李曼菲,杜何为,刘睿..玉米ECT家族成员的全基因组分析及非生物胁迫下的响应表达[J].核农学报,2024,38(6):1035-1047,13.基金项目
国家自然科学基金面上项目(32072069),湖北省自然科学基金面上项目(2022CFB183),创新群体项目(2022CFA030) （32072069）
