质膜H+-ATPase基因对铝胁迫下水稻硝态氮吸收的调控作用OA北大核心CSTPCD

To explore the regulation of nitrate nitrogen absorption(NO3--N)by the cell plasma membrane ATP enzyme(PM H+-ATPase)gene in rice under aluminum(Al)stress,two rice varieties,Feng 1A and Feng 1A super 5,were studied and seedlings without Al treatment were used as control.The activities of PM H+-ATPase hydrolysis and H+-pump,H+ efflux,the expression levels of PM H+-ATPase gene(OsA1-OsA10),the level of interaction of PM H+-ATPase and 14-3-3 protein were analysed.The results showed that,the activities of PM H+-ATPase hydrolysis and H+-pump,H+ efflux,the level of interaction of PM H+-ATPase and 14-3-3 protein showed a decrease compared with the control.The expression of gene OsA1,OsA5,OsA7 and OsA8 were suppressed.The expression level of OsA7 in Feng 1A and Feng 1A super 5 under Al stress were only 21%and 39%of the control,respectively,and the difference is significant.The uptake of NO3--N of two rice varieties are 81%and 85%of the control,respectively.In conclusion,the expression level of PM H+-ATPase gene affected the absorption capacity of NO3--N in rice,OsA1,OsA5,OsA7 and OsA8 could play a key regulatory role in the process of NO3--N absorption under Al stress.This study provides a theoretical basis for enhancing the ability of rice to absorb NO3--N under acidic Al conditions,thereby promoting their growth and yield.