基础医学与临床2024，Vol.44Issue(5)：619-625,7.DOI:10.16352/j.issn.1001-6325.2024.05.0619
RNA结合蛋白RBPMS调控急性髓系白血病细胞系THP-1增殖与迁移
RNA-binding protein RBPMS regulates proliferation and migration of acute myeloid leukemia cells line THP-1
摘要
Abstract
Objective To investigate the impact of the RNA-binding protein with multiple splicing(RBPMS),which has multiple splicing functions,on the function of acute myeloid leukemia cells line THP-1 carrying the MLL-AF9 fusion gene.Methods The expression of RBPMS in the hematopoietic system was analyzed using the GEO database;RBPMS over-expression in THP-1 cells was achieved through lentiviral infection.The efficiency was detected by RT-qPCR and Western blot;The effect of RBPMS over-expression on the THP-1 cell transcriptome was evaluated by high-throughput sequencing.The impact of RBPMS over-expression on the proliferation and migration of leukemia cells was examined using CCK-8 assay and flow cytometry,respectively.Results RBPMS expressed in acute myeloid leukemia stem cells carrying the MLL-AF9 fusion gene was found to be inhibited.Over-expression of RBPMS significantly reduced the proliferation and migration of THP-1 cells.Conclusions RBPMS inhibits cell proliferation and migration of acute myeloid leukemia cells line carrying the MLL-AF9 fusion gene.关键词
RBPMS/MLL-AF9/急性髓系白血病/急性髓系白血病细胞系(THP-1)Key words
RBPMS/MLL-AF9/acute myeloid leukemia/acute monocytic leukemia cells line(THP-1)分类
医药卫生引用本文复制引用
赵羚延,何浏,马艳妮,余佳..RNA结合蛋白RBPMS调控急性髓系白血病细胞系THP-1增殖与迁移[J].基础医学与临床,2024,44(5):619-625,7.基金项目
国家自然科学基金(82122005) （82122005）
