RNA结合蛋白RBPMS调控急性髓系白血病细胞系THP-1增殖与迁移OACSTPCD
RNA-binding protein RBPMS regulates proliferation and migration of acute myeloid leukemia cells line THP-1
目的 研究具有多重剪接功能的RNA结合蛋白(RBPMS)对携带MLL-AF9融合基因的急性髓系白血病细胞系THP-1功能的影响.方法 使用GEO数据库分析造血系统中RBPMS的表达情况;通过慢病毒感染THP-1细胞,RT-qPCR和Western blot检测RBPMS过表达效率;通过高通量测序检测RBPMS过表达后对THP-1细胞转录组的影响.分别用CCK-8法和流式细胞仪检测RBPMS过表达对白血病细胞增殖以及迁移能力的影响.结果 RBPMS在携带MLL-AF9融合基因的急性髓系白血病干细胞中异常低表达.RBPMS过表达后THP-1细胞的增殖和迁移能力显著降低(P＜0.000 1,P＜0.05).结论 RBPMS可以抑制携带MLL-AF9融合基因的急性髓系白血病细胞系的细胞增殖与迁移.
Objective To investigate the impact of the RNA-binding protein with multiple splicing(RBPMS),which has multiple splicing functions,on the function of acute myeloid leukemia cells line THP-1 carrying the MLL-AF9 fusion gene.Methods The expression of RBPMS in the hematopoietic system was analyzed using the GEO database;RBPMS over-expression in THP-1 cells was achieved through lentiviral infection.The efficiency was detected by RT-qPCR and Western blot;The effect of RBPMS over-expression on the THP-1 cell transcriptome was evaluated by high-throughput sequencing.The impact of RBPMS over-expression on the proliferation and migration of leukemia cells was examined using CCK-8 assay and flow cytometry,respectively.Results RBPMS expressed in acute myeloid leukemia stem cells carrying the MLL-AF9 fusion gene was found to be inhibited.Over-expression of RBPMS significantly reduced the proliferation and migration of THP-1 cells.Conclusions RBPMS inhibits cell proliferation and migration of acute myeloid leukemia cells line carrying the MLL-AF9 fusion gene.
赵羚延;何浏;马艳妮;余佳
中国医学科学院基础医学研究所北京协和医学院基础学院生物化学与分子生物学系,北京 100005
临床医学
RBPMSMLL-AF9急性髓系白血病急性髓系白血病细胞系(THP-1)
RBPMSMLL-AF9acute myeloid leukemiaacute monocytic leukemia cells line(THP-1)
《基础医学与临床》 2024 (005)
619-625 / 7
国家自然科学基金(82122005)
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