井冈山大学学报（自然科学版）2024，Vol.45Issue(2)：73-79,7.DOI:10.3969/j.issn.1674-8085.2024.02.010
粉葛再生体系建立的研究
THE ESTABLISHMENT OF THE REGENERATION SYSTEM OF PUERARIA THOMSONII BENTH
摘要
Abstract
In order to establish an efficient tissue culture regeneration system of Pueraria thomsonii Benth,the effects of different disinfection methods,basic medium,sucrose concentration and explants on the primary culture,secondary culture and rooting culture of P.thomsonii were studied.The results showed that the survival rate of explants was the highest when disinfected with 75%alcohol for 30 s + 0.1%mercury + Tween-80 for 8 min,reaching 83.33%.The best part of the explants was the stem segment with buds,the average plant height could reach 2.13 cm,and the reproduction coefficient was 2.MS was the best basic medium with a reproductive coefficient of 1.26,sucrose concentration was the best,the average plant height was 1.69 cm,the reproductive coefficient was 1.55,the optimal primary medium was MS + agar 5.5 g/L+ sucrose 30 g/L + 6-BA0.2 mg/L + IBA0.6 mg/L,the average plant height was 3.13 cm,the reproductive coefficient was 3.17,and the optimal submedium was MS + agar 5.5g/L+ sucrose 30 g/L + 6-BA0.6 mg/L+ IBA0.8 mg/L+ KT0.2 mg/L,the average plant height was 1.86 cm,the reproduction coefficient was 3.44,and the optimal rooting medium was MS + agar 5.5 g/L + sucrose 30g/L + IBA1.0 mg/L,the rooting rate was 100%,and the average number of taproots was 7.1.The regeneration system of P.thomsonii was established by optimizing tissue culture conditions.关键词
粉葛/植物组织培养/再生体系Key words
Pueraria thomsonii benth/plant tissue culture/regeneration system分类
农业科技引用本文复制引用
刘慧敏,高青海,陈甦,李帅,罗辉..粉葛再生体系建立的研究[J].井冈山大学学报（自然科学版）,2024,45(2):73-79,7.基金项目
安徽省科技重大专项(202003b06020029) （202003b06020029）
滁州市重点研究开发类项目(2022ZN005) （2022ZN005）
