miR-let-7c-5p对膀胱癌细胞恶性行为、血管管腔形成及HMGB2的调控作用OACSTPCD

Objective To investigate the effects of miR-let-7c-5p on the malignant behavior,vascular lumen formation and high mobility group protein(HMGB)2 in bladder cancer cells.Methods Human bladder canc-er UM-UC-3 cells were divided into routine culture(blank group),transfected with mimics-NC(mimics-NC group),transfected with miR-let-7c-5p-mimics(miR-let-7c-5p-mimics group),transfected with inhibitor-NC(inhibitor-NC group)and transfected with miR-let-7c-5p-inhibitor(miR-let-7c-5p-inhibitor group).Real-time fluorescent quantitative polymerase chain reaction was used to detect the transfection success rate of miR-let-7c-5p in each group.Transwell assay was used to detect cell invasion ability of each group.Migration ability of each group was detected by scratch test.Angiogenesis of cells in each group was detected by Matrigel tube for-mation test.The protein expression levels of HMGB2,vascular endothelial growth factor(VEGF)and glucose transporter-1(GLUT-1)were detected by Western blot.Luciferase assay was used to verify the regulatory effect of miR-let-7c-5p on HMGB2.Results There was no significant difference in the expression level of miR-let-7c-5p mRNA,invasion number,scratch healing rate,number of vascular lumen,and protein levels of HMGB2,VEGF,and GLUT-1 among the blank group,mimics-NC group,and inhibitor-NC group(P>0.05).Compared with the blank group,mimics-NC group and inhibitor-NC group,the miR-let-7c-5p mRNA expres-sion level in the miR-let-7c-5p-mimics group was increased,the invasion number,scratch healing rate,the number of vascular lumen,and the protein expression levels of HMGB2,VEGF and GLUT-1 were decreased.In the miR-let-7c-5P-inhibitor group,the miR-let-7c-5p mRNA expression level was significantly decreased,while the invasion number,scratch healing rate,the number of vascular lumen,and the protein expression lev-els of HMGB2,VEGF,and GLUT-1 were significantly increased(P<0.05).Luciferase assay showed that the level of HMGB2-Wt in the miR-let-7c-5p-mimics group was significantly lower than that in the mimics-NC group(P<0.05).Conclusion Up-regulation of miR-let-7c-5p can significantly inhibit the invasion,metasta-sis and angiogenesis of bladder cancer cells,and the mechanism is related to the inhibition of HMGB2 protein expression.