HLA-G 3'UTR基因多态性及血清可溶性HLA-G水平与儿童诺如病毒感染的相关性研究OACSTPCD

Objective To investigate the association of human leukocyte antigen-G(HLA-G)3'untranslat-ed region(3'UTR)gene polymorphism(SNP)and serum soluble HLA-G(sHLA-G)levels with norovirus infection in children.Methods A total of 346 children with norovirus infection admitted to the First Affiliated Hospital of Xinxiang Medical University from January 2021 to December 2022 were selected as the case group,and 320 healthy children who underwent physical examination during the same period were selected as the control group.Whole blood samples of all subjects were collected and DNA was extracted.HLA-G 3'UTR gene was amplified by polymerase chain reaction(PCR)and the products were sent out for sequencing.SNPstats online analysis software was used to calculate the genotype and allele frequency distribution of SNP sites between the two groups.Enzyme-linked immunosorbent assay was used to detect serum sHLA-G levels in case group and control group.Results There was no significant difference in HLA-G 3'UTR 14 bp +/-and +3142 C/G genotypes and allele frequencies between the case group and the control group(P>0.05).The serum level of sHLA-G in the case group was significantly higher than that in the control group(P<0.05).There was no significant difference in serum sHLA-G levels between groups with different degrees of no-rovirus infection(P>0.05).There was no significant difference in serum sHLA-G levels corresponding to 14 bp+/-genotype and +3142 C/G genotype between case group and control group(P>0.05).Conclusion HLA-G 3'UTR SNP is not associated with susceptibility to norovirus infection.Serum sHLA-G level has a good predic-tive value for the early diagnosis of norovirus infection,and may be a new serum biomarker.The association with the severity and prognosis of patients needs to be further verified.