检验医学与临床2024，Vol.21Issue(8)：1059-1062,1068,5.DOI:10.3969/j.issn.1672-9455.2024.08.008
病毒性脑膜炎患儿血清和脑脊液中IP-10和TNF-α的表达情况及临床意义
Expression and clinical significance of IP-10 and TNF-α in serum and cerebrospinal fluid of children with viral meningitis
摘要
Abstract
Objective To investigate the expression and clinical significance of tumor necrosis factor-α(TNF-α)and recombinant human interferon-inducible protein-10(IP-10)in serum and cerebrospinal fluid of children with viral meningitis.Methods A total of 100 children hospitalized for acute central nervous system infection in the Third Department of Pediatrics of Xingtai People's Hospital from July 2019 to December 2020 were selected.According to the type of meningitis infection,the patients were divided into viral meningitis group(52 cases),purulent meningitis group(34 cases)and tuberculous meningitis group(14 cases).Enzyme-linked immunosorbent assay was used to detect the levels of TNF-α and IP-10 in serum and cerebrospinal fluid of all subjects.Pearson correlation analysis was used to analyze the correlation between TNF-α and IP-10 lev-els in serum and cerebrospinal fluid of children with viral meningitis.The receiver operating characteristic(ROC)curve was drawn to evaluate the diagnostic value of TNF-α and IP-10 in serum and cerebrospinal fluid for the occurrence of viral meningitis.Results The levels of TNF-α and IP-10 in serum and cerebrospinal fluid in the tuberculous meningitis group were higher than those in the purulent meningitis group and the viral meningitis group,and the purulent meningitis group were higher than the viral meningitis group,and the differences were statistically significant(P<0.05).There was a significant positive correlation between the levels of IP-10 and TNF-α in serum of children with viral meningitis(r=0.313,P<0.05).There was a sig-nificant positive correlation between the levels of TNF-α and IP-10 in cerebrospinal fluid of children with viral meningitis(r=0.455,P<0.05).The results of ROC curve analysis showed that the area under the curve(AUC)of serum IP-10 and TNF-α in the diagnosis of viral meningitis were 0.887 and 0.898 respectively,which were less than 0.958 of the combination of the two indicators(Z=2.010,2.048,P<0.05),and the AUC of cerebrospinal fluid IP-10 and TNF-α in the diagnosis of viral meningitis were 0.926 and 0.908 respec-tively,which were less than 0.964 of the combination of the two indicators in the diagnosis of viral meningitis(Z=2.208,2.260,P<0.05).Conclusion The levels of TNF-α and IP-10 in serum and cerebrospinal fluid of children with viral meningitis reduced significantly,and the combined detection of TNF-α and IP-10 has impor-tant clinical value in the diagnosis of viral meningitis.关键词
病毒性脑膜炎/脑脊液/感染/肿瘤坏死因子-α/重组人干扰素诱导蛋白-10Key words
viral meningitis/cerebrospinal fluid/infection/tumor necrosis factor-α/recombinant human interferon-inducible protein-10分类
医药卫生引用本文复制引用
张娜,李静,闫晓静,张岚,刘伟霄..病毒性脑膜炎患儿血清和脑脊液中IP-10和TNF-α的表达情况及临床意义[J].检验医学与临床,2024,21(8):1059-1062,1068,5.基金项目
河北省医学科学研究计划(20201586) （20201586）
邢台市重点研发计划自筹项目(2021ZC138). （2021ZC138）
