林产化学与工业2024，Vol.44Issue(2)：27-32,6.DOI:10.3969/j.issn.0253-2417.2024.02.004
银杏种仁分离蛋白水解产物的制备及其抗氧化活性研究
Preparation of Hydrolysates from Ginkgo biloba Seed Protein Isolate and Their Antioxidant Activity
摘要
Abstract
Ginkgo biloba seed protein isolate(GBSPI)was hydrolyzed by alcalase,bromelain,and Aspergillus oryzae protease,and the antioxidant activity of the hydrolysates was evaluated through in vitro DPPH radical,hydroxyl radical,and superoxide anion radical scavenging experiments.The results showed that the yields of hydrolysates by alcalase,bromelain,and Aspergillus oryzae protease were 93.60%,60.00%and 46.80%,with degrees of hydrolysis were 13.76%,6.28%and 5.55%,respectively.The hydrolysates from three enzymes exhibited good inhibitory effects on DPPH radical,hydroxyl radical,and superoxide anion radical.Among them,the hydrolysate from alcalase showed the strongest antioxidant activity,with IC50 values of 5.29 g/L for DPPH radical,0.35 g/L for hydroxyl radical,and 7.01 g/L for superoxide anion radical,respectively.关键词
银杏种仁/水解产物/水解度/抗氧化活性Key words
Ginkgo biloba seed/hydrolysate/hydrolysis degree/antioxidant activity分类
化学化工引用本文复制引用
王翔,张彩虹,蒋建新,谢普军,黄立新..银杏种仁分离蛋白水解产物的制备及其抗氧化活性研究[J].林产化学与工业,2024,44(2):27-32,6.基金项目
江苏省农业科技创新基金(CX(22)3056) （CX(22）
