辽宁农业科学Issue(2)：1-8,8.DOI:10.3969/j.issn.1002-1728.2024.02.001
滑菇种质资源评价及遗传多样性分析
Genetic Diversity Analysis of Pholiota nameko Strains Based on ISSR and Agronomic Phenotype
摘要
Abstract
Utilizing 26 strains of Pholiota nameko as experimental materials and the ISSR molecular marker technique,we conducted a genetic relationship study on the germplasm resources of Pholiota nameko at both the cellular and molecular levels using 11 ISSR-PCR reaction primers.We also carried out the correlation a-nalysis,principal component analysis,and cluster analysis on the phenotypic traits of nine types of Pholiota nameko.The results showed that the 26 strains of the test strains were classified into five groups at the similar-ity level of 0.76.The agronomic phenotypic correlation analysis showed that there were six groups of traits with significant or highly significant correlation coefficients,suggesting that most phenotypic traits influence each other.The principal component analysis showed that the first five principal components accounted for 87.366%of the cumulative contribution rate,indicating that these five principal components can reflect the basic characteristics of the nine phenotypic traits of the Pholiota nameko.From the perspective of characteristic values and contribution rates,we found that mushroom stem length,mushroom color,the number of mushroom buds,yield,and cap thickness are the main factors causing phenotypic differences in the germplasm of Pholio-ta nameko.The analysis of phenotypic traits of the germplasm resources indicated that when the genetic dis-tance was set at 10,the 26 test strain be clustered into five groups.Our findings set the stage for future devel-opment and utilization,and provide a basis for the selection and breeding of new varieties of Pholiota nameko strains.关键词
滑菇/ISSR/聚类分析/农艺学表型Key words
Pholiota nameko/ISSR/Cluster analysis/Agronomic phenotype分类
农业科技引用本文复制引用
肇莹,马晓颖,陈珣,龚娜,刘国丽,肖军..滑菇种质资源评价及遗传多样性分析[J].辽宁农业科学,2024,(2):1-8,8.基金项目
辽宁省"揭榜挂帅"科技攻关项目(2021JH1/10400035) （2021JH1/10400035）
