畜牧与饲料科学2024，Vol.45Issue(2)：95-102,8.DOI:10.12160/j.issn.1672-5190.2024.02.012
新疆维吾尔自治区阿克苏地区某屠宰场羊源大肠杆菌MLST分型、耐药性分析和毒力基因检测
Multilocus Sequence Typing(MLST),Antimicrobial Resistance Analysis and Virulence Gene Detection of Escherichia coli Isolated from Sheep in a Slaughterhouse in Aksu Prefecture,Xinjiang Uygur Autonomous Region
摘要
Abstract
[Objective]The aim of this study was to understand the prevalence as well as antimicrobial resistance and virulence gene distribution of Escherichia coli in sheep in a slaughterhouse in Aksu Prefecture,Xinjiang Uygur Autonomous Region.[Method]A total of 30 samples of lymph node,carcass swabs and feces were collected from the healthy sheep in a slaughterhouse in Aksu Prefecture.Escherichia coli was isolated using selective culture medium,and subsequently molecularly identified using Escherichia coli specific phoA gene.Multilocus sequence typing(MLST)of the isolates was performed by PCR amplification and sequencing.K-B disk diffusion method was used for antimicrobial susceptibility test of the isolates.The presence of the antimicrobial resistance and virulence genes of the isolates were detected by PCR assay.The 96-well plate micro-broth method was used to determine the biofilm formation ability of the isolates.[Result]A total of 14 strains of Escherichia coli were isolated from the 30 samples,with a separation rate of 46.67%.The 14 isolates belonged to 10 MLST types.High resistance rates to sulfamethoxazole-trimethoprim(100%),penicillin(85.71%)and tetracycline(50.00%)were observed among the isolates.Nine isolates(64.29%)expressed multi-drug resistance phenotype.Twelve antimicrobial resistance genes,including blaTEM(85.71%),aph(3')(71.43%),tetA(64.29%),sul1(57.14%)and sul2(57.14%)were detected in the 14 isolates,and 8 isolates(57.14%)harboured the typeⅠintegron int-Ⅰgene.Ten virulence genes,including fimH(100%),yijP(92.86%),mat(92.86%),sheA(92.86%),stx1(35.71%)and ibeB(28.57%)were detected in the 14 isolates.Ten isolates(71.43%)had the ability to form biofilms.[Conclusion]The sheep derived Escherichia coli strains in the slaughterhouse had abundant intraspecific subtypes,increased antimicrobial resistance and the ability to form biofilms.They also carried multiple antimicrobial resistance and virulence genes,and thus posed a potential threat to animal food safety.关键词
大肠杆菌/MLST分型/耐药性/毒力基因/生物被膜Key words
Escherichia coli/MLST/antimicrobial resistance/virulence gene/biofilm分类
农业科技引用本文复制引用
李广西,韩婷婷,吴自豪,吴静..新疆维吾尔自治区阿克苏地区某屠宰场羊源大肠杆菌MLST分型、耐药性分析和毒力基因检测[J].畜牧与饲料科学,2024,45(2):95-102,8.基金项目
新疆生产建设兵团第九师科技计划项目"牛羊重要细菌性传染病的综合防治与示范"(2021JS001). （2021JS001）
