畜牧与饲料科学2024，Vol.45Issue(2)：116-121,6.DOI:10.12160/j.issn.1672-5190.2024.02.015
柯坪县规模化养殖场双峰驼隐孢子虫感染情况调查与种类鉴定
Infection Status and Species Identification of Cryptosporidium in Bactrian Camels in Large-scale Farms in Kalpin County
摘要
Abstract
[Objective]This study was conducted to understand the infection status and species distribution of Cryptosporidium in Bactrian camels in large-scale farms in Kalpin County,Aksu Prefecture,Xinjiang Uygur Autonomous Region.[Method]A total of 516 fecal samples were collected from 6 large-scale Bactrian camel farms in 4 townships or towns of Kalpin County,and were then subjected to genomic DNA extraction.PCR assay was carried out targeting the SSU rDNA of Cryptosporidium.After sequence alignment analysis,Cryptosporidium species were identified.The χ2 test was used to statistically compare the infection rates of Cryptosporidium in Bactrian camels among different farms.DNA samples identified as Cryptosporidium parvum(C.parvum)positive were used to perform PCR assay targeting the gp60 gene.C.parvum subtypes were determined following sequencing alignment analysis.[Result]Out of the 516 fecal DNA samples of Bactrian camels,34 samples were positive for Cryptosporidium,with an overall infection rate of 6.59%(34/516).The NO.2 farm in Aqial Town had the highest infection rate of 9.57%(9/94).There were statistically significant differences(χ2=5.497,P<0.05)in the infection rates of Cryptosporidium in Bactrian camels from different farms.Three species of Cryptosporidium,including Cryptosporidium andersoni(n =29),Cryptosporidium rat genotypeⅣ(n=1)and C.parvum(n=4),were identified in the 34 samples positive for Cryptosporidium by the comparison analysis on SSU rDNA sequence.In addition,the 4 samples positive for C.parvum were identified as subtypeⅠd-like-A21 by the comparison analysis on the gp60 gene sequence,exhibiting unique genetic evolution characteristic.[Conclusion]A low infection rate of Cryptosporidium in Bactrian camels in Kalpin County was observed,and the subtype of the infected C.parvum was unique.The results obtained in this study offered fundamental data for characterizing the species distribution and genetic evolution of Cryptosporidium in Bactrian camels in China.关键词
隐孢子虫/感染/种类鉴定/双峰驼Key words
Cryptosporidium/infection/species identification/Bactrian camel分类
农业科技引用本文复制引用
啜力文,杨彬,曹梦雅,齐萌,赫永强,司俊飞,井波,张振杰..柯坪县规模化养殖场双峰驼隐孢子虫感染情况调查与种类鉴定[J].畜牧与饲料科学,2024,45(2):116-121,6.基金项目
新疆生产建设兵团重点领域科技攻关计划项目(2020AB025). （2020AB025）
