超高效液相色谱-串联质谱法测定皮革中9种双酚类物质OA北大核心CSTPCD

A method for simultaneous determination of 9 bisphenols in leather was established by ultra-high performance liquid chromatography-tandem mass spectrometry.The sample was ultrasonically extracted with methanol.The obtained extract was purified by solid phase extraction column and concentrated to constant volume.The filtrated solution was separated by Agilent ZORBAX Eclipse Plus C18 chromatographic column(100 mm×3.0 mm,3.5 μm),using water-methanol as mobile phases for gradient elution.Multi-reaction monitoring analysis was performed using a quadrupole mass spectrometer,and internal standard method was adopted for quantification.The results showed that the 9 bisphenols could be separated within 9 min,and the good linear relationships were obtained with correlation coefficients R2 all above 0.999 0.The limits of detection and quantification were in the ranges of 0.8-24 μg/kg and 4.0-80 μg/kg,respectively.The average recoveries were 85.2％-105.6％with precisions of 2.6％-10.3％.Bisphenol F,bisphenol S,bisphenol A,bisphenol E and bisphenol AF were detected in the leathers for shoes,with the higher detection rates for bisphenol F and bisphenol S,respectively.With the advantages of convenient operation,high sensitivity and good precision,the method provides a technical support for risk monitoring of bisphenols in leather and leather goods.