皮革科学与工程2024，Vol.34Issue(3)：58-65,8.DOI:10.19677/j.issn.1004-7964.2024.03.009
超高效液相色谱-串联质谱法测定皮革中9种双酚类物质
Determination of 9 Bisphenols in Leather by Ultra-High Perfor-mance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A method for simultaneous determination of 9 bisphenols in leather was established by ultra-high performance liquid chromatography-tandem mass spectrometry.The sample was ultrasonically extracted with methanol.The obtained extract was purified by solid phase extraction column and concentrated to constant volume.The filtrated solution was separated by Agilent ZORBAX Eclipse Plus C18 chromatographic column(100 mm×3.0 mm,3.5 μm),using water-methanol as mobile phases for gradient elution.Multi-reaction monitoring analysis was performed using a quadrupole mass spectrometer,and internal standard method was adopted for quantification.The results showed that the 9 bisphenols could be separated within 9 min,and the good linear relationships were obtained with correlation coefficients R2 all above 0.999 0.The limits of detection and quantification were in the ranges of 0.8-24 μg/kg and 4.0-80 μg/kg,respectively.The average recoveries were 85.2％-105.6％with precisions of 2.6％-10.3％.Bisphenol F,bisphenol S,bisphenol A,bisphenol E and bisphenol AF were detected in the leathers for shoes,with the higher detection rates for bisphenol F and bisphenol S,respectively.With the advantages of convenient operation,high sensitivity and good precision,the method provides a technical support for risk monitoring of bisphenols in leather and leather goods.关键词
液相色谱-串联质谱/皮革/双酚/内分泌干扰物Key words
liquid chromatography-tandem mass spectrometry/leather/bisphenols/endocrine disrupting compounds分类
轻工纺织引用本文复制引用
王宁,周雷,章宦胜,胡秀红,苗洁..超高效液相色谱-串联质谱法测定皮革中9种双酚类物质[J].皮革科学与工程,2024,34(3):58-65,8.基金项目
浙江省市场监督管理局科研计划项目(ZC2021B067) （ZC2021B067）
温州市科研项目(S20220028) （S20220028）
