青岛大学学报（自然科学版）2024，Vol.37Issue(1)：1-7,7.DOI:10.3969/j.issn.1006-1037.2024.01.01
piRNA DQ725559通过抑制心肌细胞铁死亡通路抗心肌缺血再灌注损伤的作用
piRNA DQ725559 Resists Myocardial Ischemia-reperfusion Injury by Inhibiting the Ferroptosis Pathway of Cardiomyocytes
摘要
Abstract
To explore the relationship between myocardial ischemia-reperfusion injury(IRI)and ferropto-sis,and to find related Piwi-interacting RNA(piRNA),myocardial cell IRI model was constructed by Hy-poxia/Reoxygenation(H/R)of primary cardiomyocytes from neonatal mice.The optimal H/R time point for inducing ferroptosis was reflected by cell survival rate.piRNA associated with ferroptosis was screened and changes of ferroptosis related indexes were detected.The results show that ferroptosis is associated with myocardial IRI after hypoxia for 18 h and reoxygenation for 6 h.Inhibition of DQ725559 expression aggravates ferroptosis and myocardial IRI,while overexpression of DQ725559 alleviates ferroptosis and myocardial IRI.This suggests that DQ725559 can play a role in the regulation of myocardial IRI by regula-ting the ferroptosis pathway of cardiomyocytes,and overexpression of DQ725559 could be a new strategy for RNA therapy of cardiovascular diseases.关键词
缺血再灌注损伤/piRNA/心肌细胞/铁死亡Key words
ischemia-reperfusion injury/piRNA/cardiomyocytes/ferroptosis分类
医药卫生引用本文复制引用
王瑞荃,陈鑫哲,王涛,王昆..piRNA DQ725559通过抑制心肌细胞铁死亡通路抗心肌缺血再灌注损伤的作用[J].青岛大学学报（自然科学版）,2024,37(1):1-7,7.基金项目
国家自然科学基金(批准号:82070313)资助. （批准号:82070313）
