中国海洋大学学报（自然科学版）2024，Vol.54Issue(5)：33-43,11.DOI:10.16441/j.cnki.hdxb.20220157
低蛋白饲料添加亮氨酸、精氨酸对大菱鲆幼鱼生长、消化、免疫及mTOR信号通路的影响
Effects of Leucine and Arginine Supplements of Low-Protein Diets on Growth,Digestion,Immunity and Target of Rapamycin Signaling Pathway of Juvenile Turbot
摘要
Abstract
To study the effects of leucine and arginine as signaling molecules in regulating the growth of turbot,juvenile turbot(initial body weight(13.50±0.19)g)were randomly assigned to four diets:50%protein(positive control),45%protein(negative control),45%protein supplemented with 1%leucine(LP+L),and 45%protein supplemented with 1%arginine(LP+R).After 56 d feeding trial,growth performance and feed utilization of juvenile turbot were measured.The results showed that addition of leucine and arginine ameliorated the reduced specific growth rate,protein efficiency and weight gain rate of turbot caused by low-protein diet.Addition of 1%leucine and 1%arginine effectively enhanced the postprandial phosphorylation of TOR,ribosomal protein S6,and the eIF4E-binding protein 1 in liver and muscle.Moreover,1%arginine increased the activities of amylase and lipase in turbot's intestines,and significantly increased the levels of serum total antioxidant capacity(T-AOC),catalase(CAT)and lysozyme(LYZ)in serum.Our results showed that adding 1%leucine or 1%arginine to low-protein diet can activate mTOR signaling pathway and effectively improve the growth performance and protein efficiency ratio,while 1%arginine could improve digestive enzyme activity,non-specific immune reaction of turbot.关键词
大菱鲆/亮氨酸/精氨酸/mTOR信号通路/低蛋白饲料Key words
turbot/leucine/arginine/mTOR signaling pathway/low-protein diet分类
农业科技引用本文复制引用
田原,刘成栋,王旋,周慧慧,麦康森,何艮..低蛋白饲料添加亮氨酸、精氨酸对大菱鲆幼鱼生长、消化、免疫及mTOR信号通路的影响[J].中国海洋大学学报（自然科学版）,2024,54(5):33-43,11.基金项目
国家重点研究发展计划项目(2018YFD0900400) （2018YFD0900400）
国家自然科学基金项目(31772860)资助 Supported by the National Key Research and Development Program of China(2018YFD0900400) （31772860）
the National Natural Science Foun-dation of China(31772860) （31772860）
