中国海洋大学学报（自然科学版）2024，Vol.54Issue(5)：52-62,11.DOI:10.16441/j.cnki.hdxb.20230078
琼枝白化特征及其附生菌群多样性分析
Characterization of Bleached Betaphycus gelatinae and Analysis of Its Epiphytic Bacterial Diversity
摘要
Abstract
In 2021 and 2022,the bleaching of Betaphycus gelatinae has caused seriously economic and ecological losses in Hainan.To clarify the characteristics and causes of the bleaching,physiological parameters and the community structure of epiphytic bacteria of healthy(BgR)and bleached B.gelatinae(BgW)were analyzed.The morphological difference,pigment content,photophysiological and physiological parameters between BgR and BgW were compared.Using high-throughput amplicon sequencing of the 16 S rRNA gene,the alpha diversity,beta diversity and structures of the epiphytic microbial communities of BgR and BgW were analyzed as well.The study revealed that cortex cells in BgW were disrupted with significant reduction of pigments and maximum photosynthetic efficiency(QY_max).The reactive oxygen species(ROS),superoxide dismutase(SOD),malondialdehyde(MDA),and proline(Pro)were significantly reduced(p＜0.05).The number of culturable epiphytic bacteria in BgW(2.76×107 cfu/g)was remarkably higher than that in BgR(3.3×104 cfu/g).The dominant bacterium in BgR was Ruegeria while that in BgW was Yoonia.The high-throughput sequencing results revealed that no significant difference existed for the alpha diversity indices between microbial communities associated with the two groups of B.gelatinae.However,the Ace,Chao and Simpson index of BgW were higher than those of BgR.Analysis of similarities demonstrated that significant differences in epiphytic microbial communities existed between BgR and BgW.A total of 253 OTUs were identified,and Hirschia,Mesoflavibacter,Aquimarina,Agaribacterium and Achromobacter were the taxa with the most significant differences in relative abundance between BgR and BgW(p＜0.001);The dominant bacterium in BgR was Achromobacter while the most dominant bacterium in BgW was Agaribacterium.Our findings featured the B.gelatinae bleaching for the first time and provided data supporting the clarification of the function of microbial community in B.gelatinae bleaching.关键词
琼枝/白化/附生细菌/多样性/生理生化/病理特征Key words
Betaphycus gelatinae/bleach/epiphytic bacterium/diversity/physiological and biochem-ical/pathological features分类
农业科技引用本文复制引用
马艺丹,唐磊,王一帆,张帅,王振东,茅云翔,莫照兰..琼枝白化特征及其附生菌群多样性分析[J].中国海洋大学学报（自然科学版）,2024,54(5):52-62,11.基金项目
国家自然科学基金项目(32060829) （32060829）
中央高校基本科研业务费专项(202064006) （202064006）
国家重点研究发展计划项目(2020YFD0901101) （2020YFD0901101）
海南省自然科学基金项目(320QN292)资助 Supported by the National Natural Science Foundation of China(32060829) （320QN292）
the Fundamental Research Funds for Central Universities(202064006) （202064006）
the National Key Research and Development Program of China(2020YFD0901101) （2020YFD0901101）
the Natural Science Foundation of Hainan Province(320QN292) （320QN292）
