中国海洋大学学报（自然科学版）2024，Vol.54Issue(5)：125-134,10.DOI:10.16441/j.cnki.hdxb.20220197
啤酒厂过滤工段废硅藻土的生物再生及回用的可行性研究
Feasibility Study on Biological Regeneration and Reuse of Spent Diatomite in Filtration Section of Brewery
摘要
Abstract
In order to solve the waste diatomite resource waste problem and avoid the defects of thermal regeneration and chemical regeneration,this paper screened out a bacterium and a fungus for waste diato-mite regeneration treatment,and preliminatively explored the protein removal and structure improve-ment of waste diatomite after the two bacteria formed compound bacteria.The two strains showed good protein removal ability in the medium,which could degrade 83％and 88％of the protein in the medium,respectively,and significantly increased the concentration of NH4+-N in the medium.It also showed ex-cellent regeneration effect in biological treatment experiment,and the protein in waste diatomite de-creased by 54.3％.The blocked pores in the waste diatomite were released,and the specific surface area and pore volume were greatly increased from 16.50 m2/g and 0.077 cm3/g to 26.21 m2/g and 0.139 cm3/g.In order to explore the recovery of its adsorption capacity and the possibility of its reuse in beer filtration process,this study used it in beer filtration process.The results show that regenerated diatomite can re-move 67％protein in beer,and reduce the turbidity of filtered beer from 0.73 EBC to 0.22 EBC.The filtering effect of protein and turbidity is close to that of new diatomite,and far better than that of waste diatomite.After filtration,the abiotic stability of beer is improved.Contrary to the significant increase in turbidity of filtered beer with waste diatomite stored for 15 days,the turbidity of filtered beer with reclaimed diatomite stored for 15 days did not change much,which is the reason for the low content of colloidal particles such as protein in beer.Comprehensive analysis shows that the compound bacteria in this study can degrade the macromolecular proteins in the waste diatomite through their own metabo-lism,release the pores on the wheel,and effectively regenerate the waste diatomite.The regenerated di-atomite also has the possibility to be reused in the beer filtration process.关键词
啤酒/废硅藻土/生物再生/蛋白质/非生物稳定性Key words
complex microorganism/regeneration/protein/beer/abiotic stability分类
资源环境引用本文复制引用
杨淑洁,田伟君,陈海宁,高慧子,赵婧..啤酒厂过滤工段废硅藻土的生物再生及回用的可行性研究[J].中国海洋大学学报（自然科学版）,2024,54(5):125-134,10.基金项目
山东省重点研究发展计划项目(2019GSF109099)资助 Supported by the Key Research and Development Plan Project in Shandong Province(2019GSF109099) （2019GSF109099）
