摘要
Abstract
This paper mainly explores the application of ELISA detection kit in the detection of Mycoplasma gallisepticum.Using serum samples,Mycoplasma gallisepticum strain and SPF chicken as experimental materials,sensitivity test,repeatability test,antibody duration test,clinical sample detection accuracy test and preservation period test were carried out respectively.The results showed that the sensitivity of ELISA kit was good,the stability of repetitive test was as expected,the antibody level in serum of chickens increased significantly within 35 days after inoculation with weak toxicity vaccine,the detection accuracy of clinical sam-ples was high,and the sensitivity and specificity of ELISA kit were good within 320 days.According to the analysis,it is feasible to use ELISA kit to detect Mycoplasma gallisepticum in serum,and it has the advantages of simple operation,low cost and high efficiency.关键词
鸡毒支原体/ELISA检测/灵敏性试验/重复性实验/抗体持续期监测Key words
Mycoplasma gallisepticum/ELISA detection/sensitivity test/repetitive test/antibody duration monitoring Mycoplas-ma gallisepticum分类
农业科技
