日用化学工业（中英文）2024，Vol.54Issue(4)：401-409,9.DOI:10.3969/j.issn.2097-2806.2024.04.005
拉曼光谱研究表面活性剂对皮肤刺激和皮肤防护的体内分子机制
In vivo molecular mechanism of surfactant-induced skin irritation and skin protection by confocal Raman spectroscopy
何一凡 1吴文海 2苏牧楠 2蒋晓龙 3刘宇红3
作者信息
- 1. 北京工商大学 轻工科学与工程学院,北京 100048
- 2. 太和康美(北京)中医研究院有限公司,北京 102401
- 3. 北京东方淼森生物科技有限公司,北京 101200
- 折叠
摘要
Abstract
To better understand the molecular mechanism of surfactant-induced skin damage and skin protection,human(skin endogenous),exogenous substances(irritant and active ingredient against irritant),and the interaction between them need to be explored simultaneously.However,there are few reports in real-time of the mechanism at molecular level on humans.Confocal Raman spectroscopy allows such investigation with in vivo,real-time,non-invasive analysis of both substances(endogenous or exogenous)in stratum corneum(SC)and the structure of SC.In this study,the relative amount and depth of transdermal absorption of sodium dodecyl sulfate(SDS)chosen as surfactant representative,and the changes in relative content and structure of skin lipids upon SDS invasion were measured qualitatively and semi-quantitatively by scanning volunteers'skin of volar forearm at the depth of 0-12 μm with confocal Roman spectrometer.It showed that SDS was absorbed in SC,causing its lipid loss and lipid order reduction.The evidence that the amount and depth of irritant are two main factors affecting the extent of stimulation in skin was provided based on human data for the first time.The third factor,which could enhance SDS-induced barrier damage called the"conduction network of stimuli"was found in humans for the first time.The results of skin interior studied by Raman spectroscopy,combined with other techniques,including 3D skin model,Skin Surface Analyzer etc.,all were in good agreement,which came to the molecular mechanism of SDS-induced skin/barrier damage and skin protection of what happened in the real world.The proposals were confirmed by the efficacy of surfactant antagonist called ASF thus designed by the fact that compared with the absence of antagonist,the damage to the skin barrier function caused by SDS could be reduced efficiently.关键词
在体研究表面活性剂皮肤刺激的分子机制/皮肤防护/共聚焦拉曼光谱/皮肤屏障功能Key words
in vivo study on molecular mechanism of skin irritation by surfactant/skin protection/confocal Raman spectroscopy/skin barrier function分类
化学化工引用本文复制引用
何一凡,吴文海,苏牧楠,蒋晓龙,刘宇红..拉曼光谱研究表面活性剂对皮肤刺激和皮肤防护的体内分子机制[J].日用化学工业（中英文）,2024,54(4):401-409,9.
