日用化学工业（中英文）2024，Vol.54Issue(4)：410-418,9.DOI:10.3969/j.issn.2097-2806.2024.04.006
负载香芹酚的小麦醇溶蛋白/果胶纳米颗粒的构建及性质表征
Preparation and characterization of carvacrol-loaded gliadin-pectin nanoparticles
摘要
Abstract
As a natural and efficient antioxidant,carvacrol has a good antibacterial and antioxidant properties.But the carvacrol has lower bioavailability than some neurotrophic substance due to its poor water solubility.To solve this problem,the gliadin/pectin-carvacrol were prepared by anti solvent method,using gliadin as the material,and pectin as a crosslinker.The effects of different mass ratios of gliadin and pectin,different mass ratios of gliadin and carvacrol,pH value,salt ion concentration and storage time on the stability of composite nanoparticles were investigated by the means of particle size analyzer and optical photographs.The results show that the particle size of the composite nanoparticles is 179.23 nm,the polydispersity index(PDI)is 0.21,and the Zeta-potential is-36.42 mV with m(gliadin)∶m(pectin)=1∶1.5.Moreover,the encapsulation efficiency is 85.23%with the m(gliadin)∶m(carvacrol)=10∶1.When the pH=3.0-9.0 and salt ion concentration is lower than 50 mmol/L,the composite nanoparticles loaded with carvacrol have a good stability,and the storage experiment shows that the system is uniform and stable within 28 days.The results of transmission electron microscopy also show that pectin and gliadin formed a stable spherical structure through the electrostatic interaction.In addition,the antioxidant experiments show that the composite nanoparticles loaded with carvacrol can effectively eliminate free radicals and prevent food oxidation,and the IC50 of DPPH radical scavenging activity is(13.21±0.81)μg/mL,which has a good application prospect in the field of food preservation and packaging.关键词
小麦醇溶蛋白/果胶/纳米颗粒/香芹酚/稳定性Key words
gliadin/pectin/nanoparticle/carvacrol/stability分类
轻工纺织引用本文复制引用
许雪儿,金绍娣,王岚..负载香芹酚的小麦醇溶蛋白/果胶纳米颗粒的构建及性质表征[J].日用化学工业（中英文）,2024,54(4):410-418,9.基金项目
盐城工业职业技术学院自然科学一般项目(yg202104) （yg202104）
江苏省产学研合作项目(BY20221368) （BY20221368）
