日用化学工业（中英文）2024，Vol.54Issue(4)：477-483,7.DOI:10.3969/j.issn.2097-2806.2024.04.014
高效液相色谱法测定洗发护发产品中18种防腐剂和去屑剂
Determination of 18 preservatives and anti-dandruff agents in shampoo and hair care products by HPLC
摘要
Abstract
A high performance liquid chromatography(HPLC)method for simultaneous determination of 18 preservatives and anti-dandruff components in cosmetics of different bases was established,and the quality of 50 batches of commercially available shampoo and hair care cosmetics was analyzed.After the extraction of samples of different bases,the samples were analyzed by HPLC and quantified by external standard method.A C18 column(4.6 mm×250 mm,5 μm)was used and an aqueous mixture of methanol and sodium dihydrogen phosphate(pH adjusted to 3.8)was selected as mobile phase for gradient elution.The flow rate was 1.0 mL/min.The column temperature was 30℃.The chromatographic separation of the 18 components was good.The linearity between peak area and mass concentration was good(r>0.998 3)in the range of 0.4-600 μg/mL,and the detection limit was 0.4-10 mg/kg.The average recoveries(n=6)were 83.1%-110.4%,the RSDs were 0.12%-7.6%,and the recoveries were good.These components remained stable within 24 h with RSDs ranging from 0.15%to 3.8%,indicative of good stability.Nine components were detected in 50 batches of samples,including pyrithione zinc,piroctone olamine,p-chloro-m-cresol,climbazole,triclosan and salicylic acid,whose content was 2 100-6 800,39-2 900,130-370,24-1 600,0.14-0.19,28-1 900 mg/kg,respectively.Benzyl chlorophenol,o-aminophenol and o-cymen-5-ol were detected,but their content was less than the limit of quantitation and thus could not be accurately quantified.Therefore,this method could be used to simultaneously determine the 18 antiseptic and anti-dandruff components in cosmetics.关键词
防腐剂/去屑剂/高效液相色谱/含量测定Key words
preservative/anti-dandruff agent/high performance liquid chromatography/determination of content分类
化学化工引用本文复制引用
祝壮飞,徐勤科,刘婷媛,孙莺,雷虹..高效液相色谱法测定洗发护发产品中18种防腐剂和去屑剂[J].日用化学工业（中英文）,2024,54(4):477-483,7.基金项目
甘肃省药品监督管理局项目(2022GSMPA0025) （2022GSMPA0025）
甘肃省科技厅项目(22YF7FA080) （22YF7FA080）
甘肃省药品监督管理局项目(2020GSMPA010) （2020GSMPA010）
甘肃省药品监督管理局项目(2021GSMPA0014) （2021GSMPA0014）
